Construction of selenium-embedded mesoporous silica with improved antibacterial activity
| Autor: | Chen Jingjing, Siyu Ni, Shirong Ni, Yuanyuan Wei, Xuexia Yang, Feng Hong |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Surface Properties chemistry.chemical_element Nanoparticle Microbial Sensitivity Tests 02 engineering and technology 01 natural sciences Cell Line Mice Selenium Minimum inhibitory concentration Colloid and Surface Chemistry X-ray photoelectron spectroscopy 0103 physical sciences Escherichia coli Animals Particle Size Physical and Theoretical Chemistry 010304 chemical physics Surfaces and Interfaces General Medicine Mesoporous silica Silicon Dioxide 021001 nanoscience & nanotechnology Anti-Bacterial Agents chemistry Transmission electron microscopy Nanoparticles Nanomedicine 0210 nano-technology Antibacterial activity Porosity Biotechnology Nuclear chemistry |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 190:110910 |
| ISSN: | 0927-7765 |
| Popis: | In this work, different concentrations of Se-incorporated mesoporous silica nanospheres (MSNs) (5Se/MSNs and 10Se/MSNs) were successfully synthesized via an in-situ one-pot method. Their physicochemical properties were characterized by X-ray diffraction (XRD), transmission electron microscopy, and X-ray photoelectron spectroscopy (XPS). The release behaviors of Se and Si were investigated in a phosphate-buffered saline (pH = 5.5, 7.4) solution (PBS). In vitro antibacterial properties of the prepared samples were evaluated with Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The cytocompatibilities of the samples were then assessed using L929 cells. Se nanoparticles were successfully loaded onto the outer and inner surfaces of hierarchical mesoporous silica. The sizes of the Se/MSNs nanoparticles were approximately 120 nm for 5Se/MSNs and 210 nm for 10Se/MSNs. The XRD and XPS results showed that Se mainly existed in the form of Se0 in the samples. The Se/MSNs exhibited stable and sustained release of both Si and Se in PBS solution. In vitro antibactericidal tests indicated that the Se/MSNs could exhibit better antibacterial activity against S. aureus than pure Se nanoparticles after 6 and 24 h of culturing. The minimal inhibitory concentration (MIC) of 10Se/MSN was 100 μg mL-1. However, the Se/MSNs exhibited no inhibitory effect on E. coli bacteria. Furthermore, all the samples exhibited excellent cell viability. These studies demonstrate initial in vitro antibacterial activity and good cytocompatibility of Se/MSNs and their potential application in antibacterial nanomedicine. |
| Databáze: | OpenAIRE |
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