Treatment of PC12 cells by nerve growth factor, dexamethasone, and forskolin. Effects on cell morphology and expression of neurotensin and tyrosine hydroxylase
| Autor: | William Rostène, Thierry Caillaud, V. Scarcériaux, Wei Yuan Xu-van Opstal, Claude Billardon |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Tyrosine 3-Monooxygenase Neuroscience (miscellaneous) Nerve Tissue Proteins Biology Cell morphology PC12 Cells Dexamethasone Cellular and Molecular Neuroscience chemistry.chemical_compound Neurochemical Catecholamines Internal medicine medicine Neurites Animals Receptors Neurotensin Neurotensin receptor Nerve Growth Factors Rats Wistar Neurotensin Forskolin Tyrosine hydroxylase Colforsin Cell Differentiation Neoplasm Proteins Rats Gene Expression Regulation Neoplastic Endocrinology Nerve growth factor Phenotype nervous system Neurology chemistry Enzyme Induction Catecholamine Female medicine.drug |
| Zdroj: | Molecular neurobiology. 10(2-3) |
| ISSN: | 0893-7648 |
| Popis: | Several lines of anatomical, neurochemical, electrophysiological, and behavioral evidence suggest the existence of physiological interactions between neurotensin (NT) and the brain dopaminergic systems. Thus, NT has been shown to exert a neuroleptic-like action and could be implicated in the pathogenesis and treatment of schizophrenia. It is thus of particular importance to develop in vitro cell culture systems as models to study such interactions. Rat adrenal pheochromocytoma PC12 cells, which expressed high levels of tyrosine hydroxylase, were used in the present study. In contrast to rat brain cells in primary cultures, PC12 cells did not express functional NT receptors. However, they were able to express both NTmRNA and NT in response to NGF, forskolin, and dexamethasone. Those neurochemical modifications furthermore may be related to changes in the morphology of the PC12 cells in response to NGF, forskolin, and dexamethasone alone or in combination. These data suggest that PC12 cells may provide a useful model to study in vitro the regulation of both catecholamine and neurotensin phenotypes. |
| Databáze: | OpenAIRE |
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