Differential biosynthesis of proteins in separated gastric mucosal cells
| Autor: | Janet M. Carrington, Andrew K. MacGill, Clive N.A. Trotman, Warren P. Tate, John R. Greenwell, Nevin Taylor |
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| Rok vydání: | 1984 |
| Předmět: |
Physiology
Cell Separation Biology In Vitro Techniques Biochemistry Cell size chemistry.chemical_compound Biosynthesis Precursor cell medicine Centrifugation Density Gradient Animals Carbon Radioisotopes Bovine serum albumin Molecular Biology Gel electrophoresis Stomach Proteins Rats Inbred Strains General Medicine Molecular biology Rats Molecular Weight Electrophoresis medicine.anatomical_structure chemistry Gastric Mucosa Protein Biosynthesis biology.protein Electrophoresis Polyacrylamide Gel Densitometry |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0305-0491 |
| Popis: | 1. Rat gastric mucosal cells were separated according to size by rate-zonal sedimentation through a medium containing a gradient of bovine albumin. 2. Numbers and mean sizes of cells indicated two main populations, 430–1000 μm 3 and 1500–2500 μm 3 , with a discontinuity between 1000 and 1500 μm 3 . 3. Gradient fractions, combined into pools of increasing cell size, synthesized radiolabelled proteins which were resolved by electrophoresis, densitometry and computation. 4. The smallest cells (430–800 μm 3 ) tended to synthesize radiolabelled proteins with the highest relative specific radioactivities. 5. The decrease in relative specific radioactivity with increase in cell size was compatible with a progression from precursor cells towards the more differentiated state. |
| Databáze: | OpenAIRE |
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