Advanced Image Acquisition and Analytical Techniques for Studies of Living Cells and Tissue Sections
Autor: | Jana Krejčí, Michal Franek, Soňa Legartová, Eva Bártová, Petra Sehnalová, Jana Suchánková, Stanislav Kozubek, Josef Večeřa, Dmitry V. Sorokin |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microscopy education.field_of_study medicine.diagnostic_test Computer science DNA repair Population DNA replication Fluorescence recovery after photobleaching Nanotechnology Computational biology Immunofluorescence Chromatin 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Förster resonance energy transfer 030220 oncology & carcinogenesis Image Processing Computer-Assisted Pathology medicine education Instrumentation |
Zdroj: | Microscopy and Microanalysis. 22:326-341 |
ISSN: | 1435-8115 1431-9276 |
Popis: | Studies on fixed samples or genome-wide analyses of nuclear processes are useful for generating snapshots of a cell population at a particular time point. However, these experimental approaches do not provide information at the single-cell level. Genome-wide studies cannot assess variability between individual cells that are culturedin vitroor originate from different pathological stages. Immunohistochemistry and immunofluorescence are fundamental experimental approaches in clinical laboratories and are also widely used in basic research. However, the fixation procedure may generate artifacts and prevents monitoring of the dynamics of nuclear processes. Therefore, live-cell imaging is critical for studying the kinetics of basic nuclear events, such as DNA replication, transcription, splicing, and DNA repair. This review is focused on the advanced microscopy analyses of the cells, with a particular focus on live cells. We note some methodological innovations and new options for microscope systems that can also be used to study tissue sections. Cornerstone methods for the biophysical research of living cells, such as fluorescence recovery after photobleaching and fluorescence resonance energy transfer, are also discussed, as are studies on the effects of radiation at the individual cellular level. |
Databáze: | OpenAIRE |
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