Bovine antibody dependent cell-mediated cytotoxicity (ADCC) effector cells
| Autor: | Hsien-Ming Peng, E. Lee Belden |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Male
Rosette Formation Neutrophils Lymphocyte Immunology Cell Population Lymphocytes Null Heterologous chemical and pharmacologic phenomena Cell Separation Biology Peripheral blood mononuclear cell Monocytes Cell Adhesion Centrifugation Density Gradient Null cell medicine Animals education Antibody-dependent cell-mediated cytotoxicity education.field_of_study Hybridomas General Veterinary Effector Immune Sera Antibody-Dependent Cell Cytotoxicity Molecular biology medicine.anatomical_structure Cattle |
| Zdroj: | Veterinary Immunology and Immunopathology. 16:157-171 |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/0165-2427(87)90015-8 |
| Popis: | ADCC effector cells from bovine blood were separated by centrifugation, adherence and rosetting techniques. Each enriched cell population, peripheral blood mononuclear cells (PBM), null lymphocytes, monocytes and neutrophils, was then examined for its capacity to mediate ADCC. Utilizing heterologous sensitizing antisera it was found that monocytes had approximately twice the ADCC activity of null lymphocytes and that neutrophils had essentially no activity. However, when homologous sensitizing antisera were used it was found that neutrophils possessed the greatest activity followed by monocytes and null cells. Results confirm the existence of an ADCC active null lymphocyte in the bovine. |
| Databáze: | OpenAIRE |
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