Morphological and Functional Changes in the Peritumoral Adipose Tissue of Colorectal Cancer Patients
| Autor: | Andrea Rossi, Simona Budui, Elena Darra, Francesco Fantin, Gloria Mazzali, Alfredo Guglielmi, Fabrizio Vinante, Guido Franceschetti, Elena Zoico, Vanni Rizzatti, Giovanni de Manzoni, Mauro Zamboni, Corrado Pedrazzani |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Pathology Colorectal cancer Endocrinology Diabetes and Metabolism Subcutaneous Fat Medicine (miscellaneous) Adipose tissue colorectal cancer Intra-Abdominal Fat 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine Humans Medicine Lipolysis Macrophage Aged Aged 80 and over Nutrition and Dietetics Adiponectin business.industry CD68 Middle Aged medicine.disease adipose tissue 030104 developmental biology adipose tissue colorectal cancer 030220 oncology & carcinogenesis Immunohistochemistry Colorectal Neoplasms business CD163 |
| Zdroj: | Obesity. 25 |
| ISSN: | 1930-739X 1930-7381 |
| Popis: | Objective The role of peritumoral adipose tissue (AT) has not been extensively studied in colorectal cancer (CRC). Methods This study was conducted in 20 male subjects undergoing elective surgery for CRC. The differences between the peritumoral visceral adipose tissue (P-VAT), visceral adipose tissue (VAT), and subcutaneous adipose tissue (SAT) of the patients were described via immunohistochemistry and molecular biology analyses. The interactions between adipocytes and a colon cancer cell line were also investigated by using an in vitro coculture system. Results The analyses revealed that adipocytes near the tumor were significantly smaller than the adipocytes from other sites. The P-VAT was preferentially infiltrated by a CD68+/CD163+/IDO- macrophage subset with a prevalent reparative inflammatory response, while the macrophages identified in VAT and SAT mainly presented inflammatory features. Furthermore, the P-VAT presented a higher expression of adiponectin compared with other sites. Morphological analysis in vitro showed that after a few days of coculture, 3T3-L1 adipocytes were reduced in number and size with an increase in lipolysis rate and dedifferentiation phenomena. Conclusions This study reveals important morphological and functional changes in the AT surrounding the tumor as an increase in lipolysis and in adiponectin-producing adipocytes, preferentially infiltrated by a macrophage subset, with prevalent reparative inflammatory response. |
| Databáze: | OpenAIRE |
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