Localization of a peripheral membrane protein: Gβγ targets Gα Z
| Autor: | Henry R. Bourne, Sonia K. Pollitt, C. Simone Fishburn |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Vesicle-associated membrane protein 8
MAP Kinase Signaling System Recombinant Fusion Proteins Mutant Palmitates CHO Cells Mitochondrion Biology Transfection Myristic Acid Membrane Lipids Cricetulus GTP-Binding Proteins Cricetinae GTP-Binding Protein gamma Subunits Animals Multidisciplinary GTP-Binding Protein beta Subunits Peripheral membrane protein Membrane Proteins Biological Transport Intracellular Membranes Compartment (chemistry) Biological Sciences Heterotrimeric GTP-Binding Proteins GTP-Binding Protein alpha Subunits Mitochondria Cell biology Membrane Amino Acid Substitution Microscopy Fluorescence Mutagenesis Site-Directed |
| Zdroj: | Proceedings of the National Academy of Sciences. 97:1085-1090 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.97.3.1085 |
| Popis: | To explore the relative roles of protein-binding partners vs. lipid modifications in controlling membrane targeting of a typical peripheral membrane protein, Gα z , we directed its binding partner, βγ, to mislocalize on mitochondria. Mislocalized βγ directed wild-type Gα z and a palmitate-lacking Gα z mutant to mitochondria but did not alter localization of a Gα z mutant lacking both myristate and palmitate. Thus, in this paradigm, a protein–protein interaction controls targeting of a peripheral membrane protein to the proper compartment, whereas lipid modifications stabilize interactions of proteins with membranes and with other proteins. |
| Databáze: | OpenAIRE |
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