External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions

Autor: Ilke Karayel-Hacioglu, Selma Mejri, Federica Monaco, Bojan Adzic, Maisa Al Ameer, Igor Djadjovski, Francisco Llorente, Ani Vodica, Hassiba Sadaoui, Miguel Ángel Jiménez-Clavero, Cristina Cano-Gómez, Natela Toklikishvili, Jelena Maksimovic Zoric, Teufik Goletić, Alejandro Brun, Fatiha El Mellouli, Elisa Pérez-Ramírez, Hermine Hovsepyan, Jeanne El Hage, Jovita Fernández-Pinero, Sayed Hassan Salem, Kurtesh Sherifi
Jazyk: angličtina
Rok vydání: 2020
Předmět:
RNA viruses
Veterinary medicine
Rift Valley Fever
Artificial Gene Amplification and Extension
Polymerase Chain Reaction
0302 clinical medicine
Medical Conditions
Veterinary virology
Bunyaviruses
Black sea
030212 general & internal medicine
Rift Valley fever
Pathology and laboratory medicine
Vaccines
Serodiagnosis
Multidisciplinary
Zoonosis
Diagnostic test
Ruminants
Medical microbiology
Veterinary Diagnostics
3. Good health
Europe
Geography
Infectious Diseases
Serology
Black Sea
Veterinary Diseases
Viruses
Medicine
Pathogens
Research Article
Veterinary Medicine
Infectious Disease Control
Science
030231 tropical medicine
Context (language use)
Genome
Viral
Research and Analysis Methods
Microbiology
03 medical and health sciences
Diagnostic Medicine
Virology
External quality assessment
medicine
Mediterranean Sea
Animals
Humans
Molecular Biology Techniques
Molecular Biology
Training curriculum
Medicine and health sciences
Organisms
Viral pathogens
Biology and Life Sciences
Viral Vaccines
Reverse Transcriptase-Polymerase Chain Reaction
Veterinary Virology
medicine.disease
Rift Valley fever virus
Microbial pathogens
Culicidae
Veterinary Science
Laboratories
Zdroj: PLoS ONE, Vol 15, Iss 9, p e0239478 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.
Databáze: OpenAIRE
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