Protective effect of dextrans on glucose oxidase denaturation and inactivation

Autor:	Melda Altikatoglu, Yeliz Basaran-Elalmis
Rok vydání:	2012
Předmět:	Protein Denaturation Time Factors Biomedical Engineering Plasma Substitutes Buffers chemistry.chemical_compound Glucose Oxidase Glucose oxidase Denaturation (biochemistry) Incubation chemistry.chemical_classification Chromatography Aqueous solution biology Dose-Response Relationship Drug Temperature Dextrans Enzyme Activation Molecular Weight Enzyme Dextran chemistry Biochemistry biology.protein Aspergillus niger Biotechnology
Zdroj:	Artificial cells, blood substitutes, and immobilization biotechnology. 40(4)
ISSN:	1532-4184
Popis:	In the present study, the stabilizing effect of dextrans as additives on the denaturation and inactivation of glucose oxidase (GOD) was investigated. Three different molecular weighted dextrans (Mw 17.5, 75, 188 kD) were used with different concentrations. Dramatically increased enzyme activities were measured after one hour of incubation of enzyme with additives between 25–40°C in water bath. Highest activity value was measured with 75 kDa molecular weighted dextran (in concentration 30% w/v) at pH 5. Dextran as an additive supplied a long shelf-life to the enzyme at 4°C. In the presence of the 75 kDa dextran, the enzyme was more stable and its activity was increased 2.7-fold at 30°C. In addition, dextran protected GOD against inactivation by a n-heptane/aqueous buffer-stirred system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::002ea8575ce3cf85a3afe639d4da5444 https://pubmed.ncbi.nlm.nih.gov/22279960 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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