Aurora Kinase A Is Involved in Controlling the Localization of Aquaporin-2 in Renal Principal Cells
| Autor: | Sandrine Baltzer, Timur Bulatov, Christopher Schmied, Andreas Krämer, Benedict-Tilman Berger, Andreas Oder, Ryan Walker-Gray, Christin Kuschke, Kerstin Zühlke, Jenny Eichhorst, Martin Lehmann, Stefan Knapp, John Weston, Jens Peter von Kries, Roderich D. Süssmuth, Enno Klussmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
actin cytoskeleton Cell Survival QH301-705.5 macromolecular substances urologic and male genital diseases Catalysis Article Inorganic Chemistry Cyclic AMP Animals AURKA AQP2 AVP cofilin-1 Gene Silencing Physical and Theoretical Chemistry Kidney Tubules Collecting Phosphorylation Biology (General) Molecular Biology Protein Kinase Inhibitors QD1-999 Spectroscopy Aurora Kinase A Cell Proliferation Aquaporin 2 Molecular Structure urogenital system Organic Chemistry General Medicine Immunohistochemistry Actins Computer Science Applications Rats Protein Transport Chemistry Cardiovascular and Metabolic Diseases Technology Platforms 570 Biowissenschaften Biologie |
| Zdroj: | International Journal of Molecular Sciences, Vol 23, Iss 763, p 763 (2022) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 763 Int J Mol Sci |
| DOI: | 10.14279/depositonce-16370 |
| Popis: | The cAMP-dependent aquaporin-2 (AQP2) redistribution from intracellular vesicles into the plasma membrane of renal collecting duct principal cells induces water reabsorption and fine-tunes body water homeostasis. However, the mechanisms controlling the localization of AQP2 are not understood in detail. Using immortalized mouse medullary collecting duct (MCD4) and primary rat inner medullary collecting duct (IMCD) cells as model systems, we here discovered a key regulatory role of Aurora kinase A (AURKA) in the control of AQP2. The AURKA-selective inhibitor Aurora-A inhibitor I and novel derivatives as well as a structurally different inhibitor, Alisertib, prevented the cAMP-induced redistribution of AQP2. Aurora-A inhibitor I led to a depolymerization of actin stress fibers, which serve as tracks for the translocation of AQP2-bearing vesicles to the plasma membrane. The phosphorylation of cofilin-1 (CFL1) inactivates the actin-depolymerizing function of CFL1. Aurora-A inhibitor I decreased the CFL1 phosphorylation, accounting for the removal of the actin stress fibers and the inhibition of the redistribution of AQP2. Surprisingly, Alisertib caused an increase in actin stress fibers and did not affect CFL1 phosphorylation, indicating that AURKA exerts its control over AQP2 through different mechanisms. An involvement of AURKA and CFL1 in the control of the localization of AQP2 was hitherto unknown. |
| Databáze: | OpenAIRE |
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