Torularhodin and torulene are the major contributors to the carotenoid pool of marine Rhodosporidium babjevae (Golubev)
| Autor: | Bjart Frode Lutnaes, Svein Kristian Stormo, Bjarne Landfald, Synnøve Liaaen-Jensen, Sigmund Sperstad |
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| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Growth medium Molecular Structure Calanus finmarchicus Rhodotorula food and beverages Bioengineering Biology beta Carotene biology.organism_classification Carotenoids Polymerase Chain Reaction Applied Microbiology and Biotechnology Torulene Yeast chemistry.chemical_compound chemistry Dry weight Phylogenetics Botany Carotenoid Chromatography High Pressure Liquid Phylogeny Copepod Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 33:269-273 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-005-0065-0 |
| Popis: | A carotenoid-producing yeast strain, isolated from the sub-arctic, marine copepod Calanus finmarchicus, was identified as Rhodosporidium babjevae (Golubev) according to morphological and biochemical characteristics and phylogenetic inference from the small-subunit ribosomal RNA gene sequence. The total carotenoids content varied with cultivation conditions in the range 66-117 microg per g dry weight. The carotenoid pool, here determined for the first time, was dominated by torularhodin and torulene, which collectively constituted 75-91% of total carotenoids under various regimes of growth. Beta-carotene varied in the range 5-23%. A high-peptone/low-yeast extract (weight ratio 38:1) marine growth medium favoured the production of torularhodin, the carotenoid at highest oxidation level, with an average of 63% of total carotenoids. In standard yeast medium (YM; ratio 1.7:1), torularhodin averaged 44%, with increased proportions of the carotenes, torulene and beta-carotene. The anticipated metabolic precursor gamma-carotene (beta,psi-carotene) constituted a minor fraction ( |
| Databáze: | OpenAIRE |
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