Co-immobilization of multi-enzyme on reversibly soluble polymers in cascade catalysis for the one-pot conversion of gluconic acid from corn straw
| Autor: | Jianzhen Li, Yingjie Su, Tingwei Jin, Mingyue Gao, Zhaoye Zhang, Xiaoxiao Yu, Guang Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Environmental Engineering Polymers Bioengineering Cellulase 010501 environmental sciences 01 natural sciences Gluconates Zea mays Hydrolysate Catalysis chemistry.chemical_compound Cascade reaction 010608 biotechnology Glucose oxidase Cellulose Waste Management and Disposal 0105 earth and related environmental sciences biology Renewable Energy Sustainability and the Environment food and beverages General Medicine Straw Enzymes Immobilized chemistry Gluconic acid biology.protein Nuclear chemistry |
| Zdroj: | Bioresource technology. 321 |
| ISSN: | 1873-2976 |
| Popis: | The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect