1.3 μm emitting SrF2:Nd3+ nanoparticles for high contrast in vivo imaging in the second biological window
Autor: | I Villa, Marta Quintanilla, Elisa Carrasco, José García Solé, Marco Pedroni, Marco Bettinelli, Carlos Jacinto, Irene Xochilt Cantarelli, A. Vedda, Daniel Jaque García, Patricia Haro Gonzalez, Blanca del Rosal, Angeles Juarranz, Fabio Piccinelli, Francisco Rodríguez, Fiorenzo Vetrone, Adolfo Speghini, Uéslen Rocha, Dirk H. Ortgies |
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Přispěvatelé: | Villa, I, Vedda, A, Cantarelli, I, Pedroni, M, Piccinelli, F, Bettinelli, M, Speghini, A, Quintanilla, M, Vetrone, F, Rocha, U, Jacinto, C, Carrasco, E, Rodríguez, F, Juarranz, Á, del Rosal, B, Ortgies, D, Gonzalez, P, Solé, J, García, D |
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Biodistribution
Fluorescence-lifetime imaging microscopy Nd3+ Materials science Infrared Carbon Nanotube Nanoparticle Nanotechnology Fluorescence Mice fluorescence imaging In vivo rare earth doped nanoparticle General Materials Science Electrical and Electronic Engineering Ag2s Quantum Dot business.industry Infrared Up-Conversion Drug-Delivery Condensed Matter Physics nanomedicine Atomic and Molecular Physics and Optics Gold Nanoparticle Autofluorescence rare earth doped nanoparticles FIS/01 - FISICA SPERIMENTALE Optoelectronics Nanomedicine Doped Laf3 Nanoparticle business Cancer-Therapy Preclinical imaging |
Popis: | Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window (1,000–1,400 nm). This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy (infrared) photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimen’s diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3+ ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also demonstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies. |
Databáze: | OpenAIRE |
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