Phylogenetic analysis of HIV-1 archived DNA in blood and gut-associated lymphoid tissue in two patients under antiretroviral therapy
| Autor: | Annie Gosselin, Jean-Pierre Routy, Hervé Fleury, Patricia Recordon-Pinson, Petronela Ancuta |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Gut-associated lymphoid tissue 030106 microbiology Short Report Proviral DNA Human leukocyte antigen Biology Microbiology Peripheral blood mononuclear cell Epitope DNA sequencing 03 medical and health sciences chemistry.chemical_compound Virology medicine Phylogenetic comparison of archived viral sequences lcsh:RC799-869 Gastroenterology Provirus Reverse transcriptase 030104 developmental biology Infectious Diseases medicine.anatomical_structure Blood chemistry HIV-1 Parasitology lcsh:Diseases of the digestive system. Gastroenterology Gut associated lymphoid tissue (GALT) DNA |
| Zdroj: | Gut Pathogens, Vol 13, Iss 1, Pp 1-6 (2021) Gut Pathogens |
| ISSN: | 1757-4749 |
| Popis: | One of the approaches to cure human immunodeficiency virus (HIV) is the use of therapeutic vaccination. We have launched the Provir/Latitude 45 study to identify conserved CTL epitopes in archived HIV-1 DNA according to the HLA class I alleles in aviremic patients under antiretroviral therapy (ART). A HIV-1 polypeptidic therapeutic vaccine based on viral sequence data obtained from circulating blood was proposed; here, our aim was to compare the proviral DNA in blood and gut-associated lymphoid tissue (GALT). Peripheral blood mononuclear cells and gut biopsies were obtained from two HIV-1 infected patients under successful antiretroviral therapy. Total DNA was extracted including the proviral DNA. The HIV-1 reverse transcriptase was sequenced in both compartments using next generation sequencing followed by single genome sequencing; phylogenetic trees were established and compared. The proviral sequences of both compartments intra-patient exhibited a very low genetic divergence while it was possible to differentiate the sequences inter-patients; single genome sequencing analysis of two couples of samples confirmed that there was no compartmentalization of the sequences intra-patient. We conclude that, considering these two cases, the proviral DNA sequences in blood and GALT are similar and that the epitope analysis of HIV-1 provirus in blood should be considered as relevant to that observed in the GALT, a hard-to-reach major compartment, and can therefore be used for therapeutic vaccine approaches. |
| Databáze: | OpenAIRE |
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