The RNA landscape of the human placenta in health and disease

Autor: D. Stephen Charnock-Jones, Pieter-Jan Volders, Sungsam Gong, Francesca Gaccioli, Lennart Martens, Gordon C. S. Smith, Emma Cook, Ulla Sovio, Paul D. W. Kirk, Justyna Dopierala, Sylvia Richardson
Přispěvatelé: Gong, Sung [0000-0001-5796-4423], Gaccioli, Francesca [0000-0001-7178-8921], Sovio, Ulla [0000-0002-0799-1105], Kirk, Paul [0000-0002-5931-7489], Richardson, Sylvia [0000-0003-1998-492X], Smith, Gordon [0000-0003-2124-0997], Charnock-Jones, Stephen [0000-0002-2936-4890], Apollo - University of Cambridge Repository, Gong, Sungsam [0000-0001-5796-4423], Dopierala, Justyna [0000-0002-8518-3349], Cook, Emma [0000-0002-6142-4443], Volders, Pieter-Jan [0000-0002-2685-2637], Kirk, Paul D. W. [0000-0002-5931-7489], Smith, Gordon C. S. [0000-0003-2124-0997], Charnock-Jones, D. Stephen [0000-0002-2936-4890], Kirk, Paul D W [0000-0002-5931-7489], Smith, Gordon C S [0000-0003-2124-0997], Charnock-Jones, D Stephen [0000-0002-2936-4890]
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Biopsy
Placenta
Datasets as Topic
General Physics and Astronomy
EFFICIENT
RNA-Seq
Transcriptome
0302 clinical medicine
ULTRASONOGRAPHY
Pre-Eclampsia
Pregnancy
Gene expression
Medicine and Health Sciences
FRAGMENTS
GENE-EXPRESSION
Genetics
Sanger sequencing
Multidisciplinary
Fetal Growth Retardation
article
Gene Expression Regulation
Developmental
food and beverages
humanities
CIRCULAR RNAS
OUTCOME PREDICTION POP
medicine.anatomical_structure
symbols
Female
Follistatin-Related Proteins
DATABASE
Science
631/114/2402
Genetics and Molecular Biology
Biology
General Biochemistry
Genetics and Molecular Biology
38/91
03 medical and health sciences
symbols.namesake
RATIO
medicine
Humans
Gene
Messenger RNA
82/58
RNA
General Chemistry
QUANTIFICATION
631/208/199
030104 developmental biology
692/699/2732/2730
General Biochemistry
FETAL-GROWTH RESTRICTION
030217 neurology & neurosurgery
Zdroj: Nature Communications, Vol 12, Iss 1, Pp 1-17 (2021)
NATURE COMMUNICATIONS
ISSN: 2041-1723
Popis: Funder: Department of Health
The placenta is the interface between mother and fetus and inadequate function contributes to short and long-term ill-health. The placenta is absent from most large-scale RNA-Seq datasets. We therefore analyze long and small RNAs (~101 and 20 million reads per sample respectively) from 302 human placentas, including 94 cases of preeclampsia (PE) and 56 cases of fetal growth restriction (FGR). The placental transcriptome has the seventh lowest complexity of 50 human tissues: 271 genes account for 50% of all reads. We identify multiple circular RNAs and validate 6 of these by Sanger sequencing across the back-splice junction. Using large-scale mass spectrometry datasets, we find strong evidence of peptides produced by translation of two circular RNAs. We also identify novel piRNAs which are clustered on Chr1 and Chr14. PE and FGR are associated with multiple and overlapping differences in mRNA, lincRNA and circRNA but fewer consistent differences in small RNAs. Of the three protein coding genes differentially expressed in both PE and FGR, one encodes a secreted protein FSTL3 (follistatin-like 3). Elevated serum levels of FSTL3 in pregnant women are predictive of subsequent PE and FGR. To aid visualization of our placenta transcriptome data, we develop a web application ( https://www.obgyn.cam.ac.uk/placentome/ ).
Databáze: OpenAIRE
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