Free Edges in Epithelial Cell Sheets Stimulate Epidermal Growth Factor Receptor Signaling
| Autor: | Ethan R. Block, Kira L. Lathrop, Rebecca S. Sullenberger, Michael A. Tolino, Jes K. Klarlund, Abigail R. Mazie, Jennifer S. Lozano |
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| Rok vydání: | 2010 |
| Předmět: |
Cell Culture Techniques
Biology Cornea 03 medical and health sciences Tissue culture Adenosine Triphosphate 0302 clinical medicine Cell Movement medicine Humans Epidermal growth factor receptor Extracellular Signal-Regulated MAP Kinases Molecular Biology Cells Cultured 030304 developmental biology Wound Healing 0303 health sciences integumentary system Epidermal Growth Factor Epithelial Cells Articles Cell Biology Epithelium Cell biology Enzyme Activation ErbB Receptors Cell Motility medicine.anatomical_structure Matrix Metalloproteinase 9 030220 oncology & carcinogenesis biology.protein Signal Transduction |
| Zdroj: | Molecular Biology of the Cell |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e09-12-1026 |
| Popis: | Epithelia tend to migrate when edges are present, for instance, after wounding or during development. Using a new tissue culture model, we found that the existence of free edges is in itself a signal that causes activation of the epidermal growth factor and cell motility. The ability of epithelia to migrate and cover wounds is essential to maintaining their functions as physical barriers. Wounding induces many cues that may affect the transition to motility, including the immediate mechanical perturbation, release of material from broken cells, new interactions with adjacent extracellular matrix, and breakdown of physical separation of ligands from their receptors. Depending on the exact nature of wounds, some cues may be present only transiently or insignificantly. In many epithelia, activation of the epidermal growth factor receptor (EGFR) is a central event in induction of motility, and we find that its continuous activation is required for progression of healing of wounds in sheets of corneal epithelial cells. Here, we examine the hypothesis that edges, which are universally and continuously present in wounds, are a cue. Using a novel culture model we find that their presence is sufficient to cause activation of the EGFR and increased motility of cells in the absence of other cues. Edges that are bordered by agarose do not induce activation of the EGFR, indicating that activation is not due to loss of any specific type of cell–cell interaction but rather due to loss of physical constraints. |
| Databáze: | OpenAIRE |
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