Neofusicoccum parvum Colonization of the Grapevine Woody Stem Triggers Asynchronous Host Responses at the Site of Infection and in the Leaves
| Autor: | Shiho Miki, Qiang Sun, Erin R. A. Galarneau, Christopher M. Wallis, Kendra Baumgartner, Daniel P. Lawrence, Rosa Figueroa-Balderas, Dario Cantu, Mélanie Massonnet |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Biology Plant Science lcsh:Plant culture 01 natural sciences stilbenoids Transcriptome 03 medical and health sciences local defense response Botany medicine botryosphaeria dieback wood decay systemic defense response lcsh:SB1-1110 MYB Gene Botryosphaeria Original Research Canker biology biology.organism_classification medicine.disease WRKY protein domain Cell biology 030104 developmental biology Shoot Reprogramming 010606 plant biology & botany |
| Zdroj: | Massonnet, M; Figueroa-Balderas, R; Galarneau, ERA; Miki, S; Lawrence, DP; Sun, Q; et al.(2017). Neofusicoccum parvum colonization of the grapevine woody stem triggers asynchronous host responses at the site of infection and in the leaves. Frontiers in Plant Science, 8. doi: 10.3389/fpls.2017.01117. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/8wx984g6 Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science Massonnet, M; Figueroa-Balderas, R; Galarneau, ERA; Miki, S; Lawrence, DP; Sun, Q; et al.(2017). Neofusicoccum parvum colonization of the grapevine woody stem triggers asynchronous host responses at the site of infection and in the leaves. Frontiers in Plant Science, 8, 207-17. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/5xw307t7 |
| ISSN: | 1664-462X |
| Popis: | © 2017 Massonnet, Figueroa-Balderas, Galarneau, Miki, Lawrence, Sun, Wallis, Baumgartner and Cantu. Grapevine trunk diseases cause important economic losses in vineyards worldwide. Neofusicoccum parvum, one of the most aggressive causal agents of the trunk disease Botryosphaeria dieback, colonizes cells and tissues of the grapevine wood, leading to the formation of an internal canker. Symptoms then extend to distal shoots, with wilting of leaves and bud mortality. Our aim was to characterize the transcriptional dynamics of grapevine genes in the woody stem and in the leaves during Neofusicoccum parvum colonization. Genome-wide transcriptional profiling at seven distinct time points (0, 3, and 24 hours; 2, 6, 8, and 12 weeks) showed that both stems and leaves undergo extensive transcriptomic reprogramming in response to infection of the stem. While most intense transcriptional responses were detected in the stems at 24 hours, strong responses were not detected in the leaves until the next sampling point at 2 weeks post-inoculation. Network co-expression analysis identified modules of co-expressed genes common to both organs and showed most of these genes were asynchronously modulated. The temporal shift between stem vs. leaf responses affected transcriptional modulation of genes involved in both signal perception and transduction, as well as downstream biological processes, including oxidative stress, cell wall rearrangement and cell death. Promoter analysis of the genes asynchronously modulated in stem and leaves during N. parvum colonization suggests that the temporal shift of transcriptional reprogramming between the two organs might be due to asynchronous co-regulation by common transcriptional regulators. Topology analysis of stem and leaf co-expression networks pointed to specific transcription factor-encoding genes, including WRKY and MYB, which may be associated with the observed transcriptional responses in the two organs. |
| Databáze: | OpenAIRE |
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