Integration of fluorescence differential path-length spectroscopy to photodynamic therapy of the head and neck tumors is useful in monitoring clinical outcome
| Autor: | A. Amelink, I. Bing Tan, Stephen C. Kanick, Dominic J. Robinson, Baris Karakullukcu, Jan B. Aans, Henricus J. C. M. Sterenborg |
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| Přispěvatelé: | Radiation Oncology, Otorhinolaryngology and Head and Neck Surgery |
| Rok vydání: | 2015 |
| Předmět: |
Materials Science (miscellaneous)
medicine.medical_treatment Biophysics General Physics and Astronomy doismetry Photodynamic therapy Pharmacokinetics Medicine Distribution (pharmacology) Photosensitizer meta-tetrahydroxyphenylchlorin Physical and Theoretical Chemistry Mathematical Physics Oxygen saturation (medicine) business.industry Physics Cancer medicine.disease Photobleaching lcsh:QC1-999 optics Blood Volume Fraction personaliszed business Nuclear medicine pharmacokinetics lcsh:Physics |
| Zdroj: | Frontiers in Physics, 3. Frontiers Media S.A. Frontiers in Physics, Vol 3 (2015) |
| ISSN: | 2296-424X |
| DOI: | 10.3389/fphy.2015.00020 |
| Popis: | Background: The use of fluorescence differential pathlength spectroscopy (FDPS) has the potential to provide real-time information on photosensitiser pharmacokinetics, vascular physiology, and photosensitizer photobleaching based dosimetry of tumors in the oral cavity receiving m-tetrahydroxyphenylchlorin (mTHPC) photodynamic therapy (PDT). Reflectance spectra can be used provide quantitative values of oxygen saturation, blood volume fraction (DVF), blood vessel diameter, and to determine the local optical properties that can be used to correct raw fluorescence for tissue absorption. Patients and methods: Twenty-seven lesions in the oral cavity, either dysplasias or cancer were interrogated using FDPS, before and immediately after the therapeutic illumination. Results: The average tumor center (TC) to normal mucosa (NM) ratio of fluorescence was 1.50 ± 0.66. mTHPC photobleaching was observed in 24 of the lesions treated. The average extent of photobleaching was 81 ± 17%. Information from FDPS spectroscopy coupled with the clinical results of the treatment identified three types of correctable errors in the application of mTHPC-PDT: Two patients exhibited very low concentrations of photosensitizer in TC, indicating an ineffective i.v. injection of photosensitiser or an erroneous systemic distribution of mTHPC. In one in tumor we observed no photobleaching accompanied by a high DVF in the illuminated tissue, suggesting that the presence of blood prevented therapeutic light reaching the target tissue. All three of the these lesions had no clinical response to PDT. In four patients we observed less than 50% photobleaching in the tumor margins, suggesting a possible geographic miss of the oncological safety margin. One patient in this group had a recurrence within 2 months after PDT even though the initial response was good. Conclusion: The integration of FDPS to clinical PDT of head and neck tumors yields data on tissue physiology, photosensitiser content, and photobleaching that can help identify treatment errors that can potentially be corrected. |
| Databáze: | OpenAIRE |
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