The inhibitor ribonucleoprotein of poly(A)-containing non-polysomal messenger ribonucleoprotein of A. salina cryptobiotic embryos
| Autor: | Herman Slegers, Etienne Piot, H Backhovens |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
chemistry.chemical_classification
Messenger RNA Size-exclusion chromatography Biophysics Ribonucleoprotein particle RNA Cell Biology Biology Biochemistry Molecular biology Messenger RNP chemistry mRNP Structural Biology iRNP Genetics Protein biosynthesis Nucleotide Protein synthesis Molecular Biology Ribonucleoprotein |
| Zdroj: | FEBS Letters. 175:16-20 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(84)80560-8 |
| Popis: | The inhibitor present in poly(A)-containing non-polysomal mRNP preparations of A. salina cryptobiotic embryos has been purified by sucrose density gradient centrifugation, gel filtration on Sephacryl S200 and ion-exchange chromatography on DEAE-cellulose. The inhibitor is a ribonucleoprotein sedimenting at 5–6 S with a M r , of ~ 84 000 ± 6000. The particle is composed of a protein with M r of 64 000 and an RNA with a length of 85 ± 2 nucleotides and is a very potent inhibitor of the translation of mRNA. The inhibition is a property of the RNA but the inhibitor ribonucleoprotein is more efficient in the inhibition of poly(A) containing mRNA than its extracted RNA. |
| Databáze: | OpenAIRE |
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