Vascular Pedicle and Microchannels: Simple Methods Toward Effective In Vivo Vascularization of 3D Scaffolds
| Autor: | Wayne A. Morrison, Habib Joukhdar, David L. Kaplan, Geraldine M. Mitchell, Lindsay Wray, Beryl Tan, Jelena Rnjak-Kovacina, Yi-Wen Gerrand |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Scaffold Silk Biomedical Engineering Neovascularization Physiologic Pharmaceutical Science Connective tissue Biocompatible Materials 02 engineering and technology Matrix (biology) 010402 general chemistry 01 natural sciences Rats Sprague-Dawley silk biomaterials Biomaterials Neovascularization angiogenesis Vascularity vascularization Tissue engineering In vivo medicine Animals Cells Cultured Tissue Engineering Tissue Scaffolds Biomaterial 021001 nanoscience & nanotechnology Immunohistochemistry vascular pedicles Rats 0104 chemical sciences microchannels medicine.anatomical_structure medicine.symptom 0210 nano-technology Biomedical engineering |
| Zdroj: | Advanced Healthcare Materials. 8:1901106 |
| ISSN: | 2192-2659 2192-2640 |
| Popis: | Poor vascularization remains a key limiting factor in translating advances in tissue engineering to clinical applications. Vascular pedicles (large arteries and veins) isolated in plastic chambers are known to sprout an extensive capillary network. This study examined the effect vascular pedicles and scaffold architecture have on vascularization and tissue integration of implanted silk scaffolds. Porous silk scaffolds with or without microchannels are manufactured to support implantation of a central vascular pedicle, without a chamber, implanted in the groin of Sprague Dawley rats, and assessed morphologically and morphometrically at 2 and 6 weeks. At both time points, blood vessels, connective tissue, and an inflammatory response infiltrate all scaffold pores externally, and centrally when a vascular pedicle is implanted. At week 2, vascular pedicles significantly increase the degree of scaffold tissue infiltration, and both the pedicle and the scaffold microchannels significantly increase vascular volume and vascular density. Interestingly, microchannels contribute to increased scaffold vascularity without affecting overall tissue infiltration, suggesting a direct effect of biomaterial architecture on vascularization. The inclusion of pedicles and microchannels are simple and effective proangiogenic techniques for engineering thick tissue constructs as both increase the speed of construct vascularization in the early weeks post in vivo implantation. |
| Databáze: | OpenAIRE |
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