Comparison of 3 Handling Techniques for Endoscopically Obtained Gastric and Duodenal Biopsy Specimens: A Prospective Study in Dogs and Cats
| Autor: | Edouard Reyes-Gomez, Guillaume Ruiz, Valérie Freiche, Edward J Hall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
040301 veterinary sciences Duodenum Biopsy Stomach Diseases Histopathology Standard Article Cat Diseases Mount Specimen Handling 0403 veterinary science 03 medical and health sciences 0302 clinical medicine Dogs Medicine Animals Dog Diseases Prospective Studies Duodenoscopy Duodenal Diseases Prospective cohort study CATS General Veterinary medicine.diagnostic_test business.industry Stomach Gastroenterology 04 agricultural and veterinary sciences Standard Articles Surgery medicine.anatomical_structure Cats 030211 gastroenterology & hepatology SMALL ANIMAL business Nuclear medicine |
| Zdroj: | Journal of Veterinary Internal Medicine |
| ISSN: | 1939-1676 0891-6640 |
| Popis: | Background Limited evidence exists in the literature regarding whether a specific mount is preferable to use for processing endoscopically obtained gastrointestinal biopsy specimens. Hypothesis/Objectives To compare 3 methods of handling endoscopically obtained gastrointestinal biopsy specimens from collection to laboratory processing and to determine if any technique produced superior results. Animals Twenty-three dogs and cats presented for gastrointestinal signs. Methods Prospective study of dogs and cats presented with gastrointestinal signs to a veterinary teaching referral hospital which underwent upper gastrointestinal endoscopy. Biopsy specimens were taken from the stomach and duodenum and submitted to the laboratory using 3 techniques: mounted on a cucumber slice, mounted on a moisturized synthetic foam sponge, and floating free in formalin. The techniques were compared with regard to the specimens' width, orientation, presence of artifacts, and pathologist's confidence in diagnosis. Results Twenty-three patients were included, with a total of 528 biopsies collected. Specimens on cucumber slice and on sponge were significantly wider (P < .001 and P = .001, respectively) compared to those floating free in formalin (mean width of 3.81 versus 3.31 and 2.52 mm, respectively). However, specimens on synthetic sponge had significantly fewer artifacts compared to those on cucumber slice (P = .05) and those floating free in formalin (P = .02). Confidence in the diagnosis also was superior with the sponge technique over floating free specimens (P = .002). Conclusions and Clinical Importance The use of mounted gastrointestinal biopsy specimens was superior over the use of specimens floating free in formalin. This technique improved the quality of the specimens and the pathologist's confidence in their histopathologic interpretation. |
| Databáze: | OpenAIRE |
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