Desiccation‐induced alterations in surface topography of thylakoids from resurrection plantHaberlea rhodopensis studied by atomic force microscopy, electrokinetic and optical measurements
| Autor: | Virjinia Doltchinkova, Tonya D. Andreeva, Katya Georgieva, Konstantin Balashev, Gergana Mihailova |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Light Physiology ved/biology.organism_classification_rank.species Resurrection plant macromolecular substances Plant Science Microscopy Atomic Force Thylakoids 01 natural sciences 03 medical and health sciences Electrokinetic phenomena Dynamic light scattering Genetics Desiccation Haberlea Lamella (cell biology) biology ved/biology Chemistry food and beverages Cell Biology General Medicine biology.organism_classification Droughts 030104 developmental biology Craterostigma 13. Climate action Thylakoid Biophysics Spinach 010606 plant biology & botany |
| Zdroj: | Physiologia Plantarum. 166:585-595 |
| ISSN: | 1399-3054 0031-9317 |
| Popis: | With their ability to survive complete desiccation, resurrection plants are a suitable model system for studying the mechanisms of drought tolerance. In the present study, we investigated desiccation-induced alterations in surface topography of thylakoids isolated from well-hydrated, moderately dehydrated, severely desiccated and rehydrated Haberlea rhodopensis plants by means of atomic force microscopy (AFM), electrokinetic and optical measurements. According to our knowledge, so far, there were no reports on the characterization of surface topography and polydispersity of thylakoid membranes from resurrection plants using AFM and dynamic light scattering. To study the physicochemical properties of thylakoids from well-hydrated H. rhodopensis plants, we used spinach thylakoids for comparison as a classical model from higher plants. The thylakoids from well-hydrated H. rhodopensis had a grainy surface, significantly different from the well-structured spinach thylakoids with distinct grana and lamella, they had twice smaller cross-sectional area and were 1.5 times less voluminous than that of spinach. Significant differences in their physicochemical properties were observed. The dehydration and subsequent rehydration of plants affected the size, shape, morphology, roughness and therefore the structure of the studied thylakoids. Drought resulted in significant enhancement of negative charges on the outer surface of thylakoid membranes which correlated with the increased roughness of thylakoid surface. This enhancement in surface charge density could be due to the partial unstacking of thylakoids exposing more negatively charged groups from protein complexes on the membrane surface that prevent from possible aggregation upon drought stress. |
| Databáze: | OpenAIRE |
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