Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications

Autor: Eva Cuypers, Tiffany Porta Siegel, Martijn Poeze, Anastasiya Svirkova, Sylvia P. Nauta, Martina Marchetti-Deschmann, Ron M. A. Heeren, Michiel Vandenbosch
Přispěvatelé: Orthopedie, RS: M4I - Imaging Mass Spectrometry (IMS), Surgery, MUMC+: NAZL en ROAZ (9), MUMC+: MA Heelkunde (9), RS: NUTRIM - R2 - Liver and digestive health, Imaging Mass Spectrometry (IMS)
Rok vydání: 2020
Předmět:
Male
Bone tissue
Forensic Medicine/methods
Wistar
01 natural sciences
Biochemistry
Gelatin
Bone and Bones/chemistry
Analytical Chemistry
Sample preparation
Microtomy/methods
0303 health sciences
Chemistry
Bio interface
Lipids
Maldi msi
Tissue Embedding/methods
medicine.anatomical_structure
Research Paper
Gelatin/chemistry
food.ingredient
Human bone
Bone and Bones
Mass spectrometry imaging
03 medical and health sciences
Lipids/analysis
food
Matrix-Assisted Laser Desorption-Ionization/methods
medicine
Animals
Rats
Wistar
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization/methods
MALDI
030304 developmental biology
Detection limit
Tissue Embedding
Spectrometry
010401 analytical chemistry
Microtomy
Mass
Forensic Medicine
Rats
Carboxymethylcellulose Sodium/chemistry
0104 chemical sciences
Carboxymethylcellulose Sodium
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Biomedical engineering
Zdroj: Analytical and Bioanalytical Chemistry, 413(10), 2683-2694. Springer
Analytical and Bioanalytical Chemistry
ISSN: 1618-2650
1618-2642
DOI: 10.1007/s00216-020-02920-1
Popis: In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples. The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue. Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular. Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively. Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials. The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning. Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue. The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples. Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated. DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode. The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue. The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle. The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios. The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations. Electronic supplementary material The online version of this article (10.1007/s00216-020-02920-1) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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