Hepatic Response of Magnesium-Restricted Wild Type Mice
| Autor: | Walter Goessler, Karine Sargsyan, Johannes Haybaeck, Tanja Macheiner, Maria Ratzer, Vera H. I. Fengler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism experimental mouse model Inflammation magnesium-deficiency Biochemistry Microbiology Article chemistry.chemical_compound Internal medicine Magnesium deficiency (medicine) Medicine Molecular Biology hepatic inflammation business.industry Cholesterol hepatic steatosis Fatty liver non-alcoholic fatty liver disease medicine.disease QR1-502 medicine.anatomical_structure Endocrinology chemistry Hepatocyte Tumor necrosis factor alpha Liver function magnesium-restriction Metabolic syndrome medicine.symptom business |
| Zdroj: | Metabolites, Vol 11, Iss 762, p 762 (2021) Metabolites Volume 11 Issue 11 |
| ISSN: | 2218-1989 |
| Popis: | Magnesium-deficiency is implicated in many metabolic disorders, e.g., type 2 diabetes and metabolic syndrome, representing risk factors for non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the contribution of magnesium-restriction to the development of NAFLD. Magnesium-deficiency was induced in C57BL/6 mice by feeding a magnesium-deficient-diet. Metabolic markers as well as markers of inflammation and liver function were assessed. Furthermore, liver tissue was examined histopathologically and compared with specimens from high-fat-diet fed and control mice. Finally, the hepatic inflammatory response was quantified by determining hepatic IL-6, TNFα, and MCP-1. Magnesium-restriction resulted in at least a 2-fold significant reduction of serum magnesium levels compared to the high-fat-diet fed and control mice, whereas the hepatic magnesium content was decreased due to high-fat-diet feeding. No changes in metabolic markers in magnesium-restricted mice were observed, while the cholesterol content was elevated in high-fat-diet fed mice. Magnesium-restricted mice additionally featured inflammation and enlarged hepatocytes in liver histology. Furthermore, magnesium-restricted and high-fat-diet fed mice exhibited elevated hepatic TNFα levels compared to control mice. Accordingly, our data suggest that magnesium is involved in hepatic inflammatory processes and hepatocyte enlargement, key histological features of human NAFLD, and may therefore contribute to development and progression of the disease. |
| Databáze: | OpenAIRE |
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