Concurrent Occurrence Of Human And Equine West Nile Virus Infections In Central Anatolia, Turkey: The First Evidence For Circulation Of Lineage 1 Viruses
| Autor: | Mahur H. Turan, Dürdal Us, Gürol Emekdaş, Feray Alkan, Seda Tezcan, Ethem Murat Arsava, Koray Ergünay, Aykut Ozkul, Sabri Hacıoğlu, Aydan Koysuren |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Microbiology (medical) Lineage (genetic) Turkey viruses 030231 tropical medicine Antibodies Viral Real-Time Polymerase Chain Reaction Virus Serology 03 medical and health sciences Flaviviridae WNV 0302 clinical medicine medicine Animals Humans Horses Clade Phylogeny Cerebrospinal Fluid 0303 health sciences biology Molecular epidemiology 030306 microbiology Meningoencephalitis virus diseases Central Anatolia General Medicine Middle Aged biology.organism_classification medicine.disease Virology 3. Good health nervous system diseases Infectious Diseases Encephalitis RNA Viral Female Horse Diseases West Nile virus West Nile Fever |
| Popis: | Summary Background West Nile fever is an important zoonotic infection caused by West Nile virus (WNV), a member of the Flaviviridae . Previous serological data from Turkey suggest widespread WNV circulation. This report includes cases of human and equine WNV infections occurring concurrently, and manifesting as central nervous system infections, in two neighboring provinces of Central Anatolia, Turkey. A partial phylogenetic analysis of the causative virus is given for the first time. Methods The cases were reported in February (horses) and March (human). Symptoms of the disease were similar in the two species, characterized by neurological manifestations suggesting meningoencephalitis. Real-time/nested PCRs and commercial immunoassays and a plaque reduction neutralization assay were employed for the detection of viral RNA and specific antibodies, respectively. Results WNV RNAs were detected in buffy coat (horses) and cerebrospinal fluid (human) samples. Partial nucleotide sequences of the E-gene coding region revealed that the strains are closely related to viruses of lineage 1, clade 1a. Accompanying equine serosurveillance demonstrated WNV-specific antibodies in 31.6% of the samples. Conclusions This is the first report of acute WNV infections caused by lineage 1 strains from Turkey, in concordance with previous reports from some European and North African countries. |
| Databáze: | OpenAIRE |
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