Association of SIRT-1 Gene Polymorphism and Vitamin D Level in Egyptian Patients With Rheumatoid Arthritis
| Autor: | Shereen Rashad Kaddafy, Ahmed A. Abdel-Aziz, Amany A. Abou-Elalla, Abdelfattah Kasem Nassar, Sadek Mostafa Sadek, Mohamed Moneer Rayan, Dina Sabry |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Single-nucleotide polymorphism Gastroenterology 03 medical and health sciences 0302 clinical medicine Polymorphism (computer science) Internal medicine Genotype Vitamin D and neurology medicine Vitamin D Rheumatoid arthritis Genotyping 030203 arthritis & rheumatology business.industry General Medicine medicine.disease Genotype frequency SIRT-1 polymorphism 030104 developmental biology Original Article Egypt Gene polymorphism business |
| Zdroj: | Journal of Clinical Medicine Research |
| ISSN: | 1918-3011 1918-3003 |
| DOI: | 10.14740/jocmr3067e |
| Popis: | Background: We investigated SIRT-1 genetic variant and its association with vitamin D level in Egyptian patients with rheumatoid arthritis (RA). Methods: Seventy Egyptian subjects were enrolled in our study and divided into two groups: RA group (n = 50 patients) and healthy control group (n = 20 subjects). Five milliliter blood sample was withdrawn from each subject followed by laboratory investigation and DNA extraction for SIRT-1 gene polymorphism assessment (rs7895833 A>G, rs7069102 C>G and rs2273773 C>T) and vitamin D level expression. Results: There was statistically significant difference between rheumatoid cases and controls with regard to vitamin D level with 88% of cases showing insufficient vitamin D versus all controls showing sufficient level. SIRT-1 different SNPs rs2273773, rs7895833and rs7069102 genotype frequencies were statistically significant in RA compared to control group (P = 0.001). There was no statistically significant difference between different genotypes of rs2273773, rs7895833 and rs7069102 with regard to vitamin D level. Conclusion: We concluded that there is a strong association between SIRT-1 polymorphism genotyping and RA. Vitamin D level was insufficient in Egyptian patients with RA. J Clin Med Res. 2018;10(3):189-195 doi: https://doi.org/10.14740/jocmr3067e |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|