Blood vessel-on-a-chip examines the biomechanics of microvasculature
| Autor: | Steven D. Hudson, Stella Alimperti, Paul Salipante |
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| Rok vydání: | 2022 |
| Předmět: |
Materials science
Membrane permeability Linear elasticity Hydrostatic pressure Mean Vessel Diameter General Chemistry Strain hardening exponent Elasticity (physics) Condensed Matter Physics Elasticity Article Biomechanical Phenomena Elastic Modulus Lab-On-A-Chip Devices Hyperelastic material Microvessels Deformation (engineering) Biomedical engineering |
| Zdroj: | Soft Matter |
| ISSN: | 1744-6848 1744-683X |
| DOI: | 10.1039/d1sm01312b |
| Popis: | We use a three-dimensional (3D) microvascular platform to measure the elasticity and membrane permeability of the endothelial cell layer. The microfluidic platform is connected with a pneumatic pressure controller to apply hydrostatic pressure. The deformation is measured by tracking the mean vessel diameter under varying pressures up to 300 Pa. We obtain a value for the Young's modulus of the cell layer in low strain where a linear elastic response is observed and use a hyperelastic model that describes the strain hardening observed at larger strains (pressure). A fluorescent dye is used to track the flow through the cell layer to determine the membrane flow resistance as a function of applied pressure. Finally, we track the 3D positions of cell nuclei while the vessel is pressurized to observe local deformation and correlate inter-cell deformation with the local structure of the cell layer. This approach is able to probe the mechanical properties of blood vessels in vitro and provides a methodology for investigating microvascular related diseases. |
| Databáze: | OpenAIRE |
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