Nasally administered cholera toxin A-subunit acts as a mucosal adjuvant
| Autor: | Hirotsugu Yamamoto, Masahiko Fukumoto, Emilio A. Campos, Masafumi Yamamoto, Satomi Maeba, Jun Namikoshi |
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| Rok vydání: | 2003 |
| Předmět: |
Cholera Toxin
Saliva Ovalbumin T-Lymphocytes Biology medicine.disease_cause Antigen-Antibody Reactions Mice Adjuvants Immunologic Antigen Antibody Specificity medicine Animals Antibody-Producing Cells Immunity Mucosal General Dentistry Administration Intranasal Mice Inbred BALB C Cholera toxin Membrane raft respiratory system Nasal Mucosa Vibrio cholerae Antibody Formation Immunology biology.protein Nasal administration Antibody |
| Zdroj: | Journal of Oral Science. 45:25-31 |
| ISSN: | 1880-4926 1343-4934 |
| DOI: | 10.2334/josnusd.45.25 |
| Popis: | It is well established that cholera toxin (CT) produced by Vibrio cholerae acts as a potent mucosal adjuvant; however, the native form of this molecule causes severe diarrhea. Furthermore, both native CT and its B-subunit derivative bind to monosialogangliosides (GM1) in membrane raft micro-domains on neural tissues and are thus unsuitable for use in humans. In this study, we evaluated the adjuvanticity of the CT A-subunit (CT-A) administered with ovalbumin (OVA) by the nasal route. We found that nasal administration of OVA plus CT-A elicited both mucosal and systemic antibody (Ab) responses. Immunization of mice with OVA plus CT-A resulted in the induction of OVA-specific IgA Abs in saliva and nasal secretions. Furthermore, significant OVA-specific serum immunoglobulin (Ig) G and IgA Ab responses were induced. Antibody-forming cell (AFC) analysis confirmed the Ab titer findings by revealing significant numbers of OVA-specific IgA AFCs in submandibular glands. In addition, splenic lymphocytes restimulated with OVA in vitro exhibited significant proliferative responses. Thus, CT-A might be a candidate for an effective adjuvant for inducing antigen (Ag)-specific Ab responses in human systemic and mucosal compartments, such as the oral cavity. |
| Databáze: | OpenAIRE |
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