Carbonic anhydrase activation is associated with worsened pathological remodeling in human ischemic diabetic cardiomyopathy

Autor: Ciro Indolfi, Clarice Gareri, Fabiola Marino, Michele Torella, Fiona C. Lewis, Daniele Torella, Iolanda Aquila, Domenico Cozzolino, Claudio Iaconetti, Beverley J. Henning, Giovanni Cuda, Nadia Lascar, Teresa Salvatore, Georgina M. Ellison, Roberto Torella, Carla Vicinanza, Mariangela Scalise, Ferdinando Carlo Sasso, Gianantonio Nappi
Přispěvatelé: Torella, Daniele, Ellison, Georgina M, Torella, Michele, Vicinanza, Carla, Aquila, Iolanda, Iaconetti, Claudio, Scalise, Mariangela, Marino, Fabiola, Henning, Beverley J, Lewis, Fiona C, Gareri, Clarice, Lascar, Nadia, Cuda, Giovanni, Salvatore, Teresa, Nappi, Gianantonio, Indolfi, Ciro, Torella, Roberto, Cozzolino, Domenico, Sasso, Ferdinando Carlo
Rok vydání: 2014
Předmět:
Blood Glucose
Male
Diabetic Cardiomyopathies
carbonic anhydrase
Myocardial Ischemia
Apoptosis
p38 Mitogen-Activated Protein Kinases
Molecular Cardiology
Muscle hypertrophy
Diabetic cardiomyopathy
Myocytes
Cardiac
Myocardial infarction
Phosphorylation
Diabetic Cardiomyopathie
Cation Transport Proteins
Cells
Cultured
Original Research
Endothelial Cell
Sodium-Hydrogen Exchanger 1
Ventricular Remodeling
microRNA
diabetes mellitu
Middle Aged
Up-Regulation
Endothelial stem cell
diabetes mellitus
Female
Cardiology and Cardiovascular Medicine
hypertrophy
Human
Signal Transduction
medicine.medical_specialty
Carbonic Anhydrase I
Sodium-Hydrogen Exchangers
Cardiomegaly
Sodium-Hydrogen Antiporter
Carbonic Anhydrase II
Internal medicine
Diabetes mellitus
medicine
Animals
Humans
Rats
Wistar
Ventricular remodeling
Aged
Ischemic cardiomyopathy
Animal
business.industry
p38 Mitogen-Activated Protein Kinase
Apoptosi
Endothelial Cells
medicine.disease
Rats
Enzyme Activation
MicroRNAs
Endocrinology
Cation Transport Protein
Diabetes Mellitus
Type 2
Heart failure
Rat
business
Zdroj: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
ISSN: 2047-9980
Popis: Background Diabetes mellitus ( DM ) has multifactorial detrimental effects on myocardial tissue. Recently, carbonic anhydrases ( CAs ) have been shown to play a major role in diabetic microangiopathy but their role in the diabetic cardiomyopathy is still unknown. Methods and Results We obtained left ventricular samples from patients with DM type 2 (DM‐T2) and nondiabetic ( NDM ) patients with postinfarct heart failure who were undergoing surgical coronary revascularization. Myocardial levels of CA ‐I and CA‐ II were 6‐ and 11‐fold higher, respectively, in DM ‐T2 versus NDM patients. Elevated CA ‐I expression was mainly localized in the cardiac interstitium and endothelial cells. CA ‐I induced by high glucose levels hampers endothelial cell permeability and determines endothelial cell apoptosis in vitro. Accordingly, capillary density was significantly lower in the DM ‐T2 myocardial samples (mean± SE =2152±146 versus 4545±211/mm 2 ). On the other hand, CA‐II was mainly upregulated in cardiomyocytes. The latter was associated with sodium‐hydrogen exchanger‐1 hyperphosphorylation, exaggerated myocyte hypertrophy (cross‐sectional area 565±34 versus 412±27 μm 2 ), and apoptotic death (830±54 versus 470±34 per 10 6 myocytes) in DM ‐T2 versus NDM patients. CA‐II is activated by high glucose levels and directly induces cardiomyocyte hypertrophy and death in vitro, which are prevented by sodium‐hydrogen exchanger‐1 inhibition. CA‐II was shown to be a direct target for repression by micro RNA ‐23b, which was downregulated in myocardial samples from DM ‐T2 patients. MicroRNA‐23b is regulated by p38 mitogen‐activated protein kinase, and it modulates high‐glucose CA‐II–dependent effects on cardiomyocyte survival in vitro. Conclusions Myocardial CA activation is significantly elevated in human diabetic ischemic cardiomyopathy. These data may open new avenues for targeted treatment of diabetic heart failure.
Databáze: OpenAIRE
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