Regulation of flavonol content and composition in (Syrah×Pinot Noir) mature grapes: integration of transcriptional profiling and metabolic quantitative trait locus analyses
| Autor: | Emanuela Coller, Claudio Moser, Urska Vrhovsek, Giulia Malacarne, Juri Battilana, Maria Stella Grando, L. Costantini, Riccardo Velasco |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Candidate gene
Flavonols QTL Physiology Molecular Sequence Data Quantitative Trait Loci Population Plant Science Microarray Biology Quantitative trait locus Segregating population Gene Expression Regulation Plant Metabolic profiling Vitis Flavonoli MYB Settore CHIM/10 - CHIMICA DEGLI ALIMENTI education Gene Plant Proteins Genetics chemistry.chemical_classification Profilo metabolico education.field_of_study Pigmentation Bacca Vitis vinifera Gene Expression Profiling food and beverages Sequence Analysis DNA Gene expression profiling Metabolic pathway Berry chemistry Popolazione segregante Gene candidato Research Paper |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/erv243 |
| Popis: | Highlight Novel candidate genes for the fine regulation of flavonol content in ripe berries are identified through integration of transcriptional profiling and metabolic QTL analyses of a segregating grapevine progeny. Flavonols are a ubiquitous class of flavonoids that accumulate preferentially in flowers and mature berries. Besides their photo-protective function, they play a fundamental role during winemaking, stabilizing the colour by co-pigmentation with anthocyanins and contributing to organoleptic characteristics. Although the general flavonol pathway has been genetically and biochemically elucidated, the genetic control of flavonol content and composition at harvest is still not clear. To this purpose, the grapes of 170 segregating F1 individuals from a ‘Syrah’×’Pinot Noir’ population were evaluated at the mature stage for the content of six flavonol aglycons in four seasons. Metabolic data in combination with genetic data enabled the identification of 16 mQTLs (metabolic quantitative trait loci). For the first time, major genetic control by the linkage group 2 (LG 2)/MYBA region on flavonol variation, in particular of tri-hydroxylated flavonols, is demonstrated. Moreover, seven regions specifically associated with the fine control of flavonol biosynthesis are identified. Gene expression profiling of two groups of individuals significantly divergent for their skin flavonol content identified a large set of differentially modulated transcripts. Among these, the transcripts coding for MYB and bZIP transcription factors, methyltranferases, and glucosyltranferases specific for flavonols, proteins, and factors belonging to the UV-B signalling pathway and co-localizing with the QTL regions are proposed as candidate genes for the fine regulation of flavonol content and composition in mature grapes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|