Preparation and characterization of the Adriamycin-loaded amphiphilic chitosan nanoparticles and their application in the treatment of liver cancer
| Autor: | Jin Han, Zi‑Ming Zhao, Hui‑Jie Zhuang, Chang‑Hua Kou, Xi‑Lin Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
algal polysaccharide myristoyl carboxymethyl chitosan
Cancer Research hepatoma-target ability antitumor drug delivery system 02 engineering and technology Pharmacology Polysaccharide 03 medical and health sciences Adriamycin 0302 clinical medicine In vivo medicine chemistry.chemical_classification Articles 021001 nanoscience & nanotechnology medicine.disease Hemolysis Acute toxicity In vitro Oncology chemistry Apoptosis 030220 oncology & carcinogenesis Drug delivery Toxicity Cancer research lactose myristoyl carboxymethyl chitosan 0210 nano-technology |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | In the present study, two nanoparticles including lactose myristoyl carboxymethyl chitosan (LMCC) and algal polysaccharide myristoyl carboxymethyl chitosan (AMCC), were obtained for hepatic-targeted Adriamycin (ADM) drug delivery systems. ADM was successfully loaded into the LMCC or AMCC nanoparticle by dialysis. The release function and liver targeting of the nanoparticles was explored, and it was revealed that ADM release from the nanoparticles was greatest at acidic pH 5.5. ADM-conjugated nanoparticles were readily taken up by HU7 human hepatocellular carcinoma cells, relative to HT22 mouse hippocampal neuron cells in vitro. In vivo, ADM-loaded nanoparticles had significant antitumor efficacy with a 62.7% inhibition rate, followed by ADM and ADM-AMCC (51.2 and 42.5%, respectively). The tissue distribution study confirmed that ADM-LMCC had an improved liver delivery efficacy, by comparison with ADM. Furthermore, a series of safety studies, including hemolysis, acute toxicity and organ toxicity, revealed that the ADM-loaded LMCC and AMCC nanoparticles had advantages over the commercially available injectable preparation of Adriamycin hydrochloride, in terms of low toxicity levels and increased tolerated dose. These results indicated that LMCC is a promising carrier for injectable ADM nanoparticle and ADM-conjugated nanoparticles may improve the efficacy of ADM by hepatic targeting. |
| Databáze: | OpenAIRE |
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