Nanofibrous Scaffolds Containing Hydroxyapatite and Microfluidic-Prepared Polyamidoamin/BMP-2 Plasmid Dendriplexes for Bone Tissue Engineering Applications
| Autor: | Fatemeh Sadat Mahdavi, Mohammad Porgham Daryasari, Iman Shabani, Ehsan Seyedjafari, Farzad Moradikhah, Mohsen Amini, Banafsheh Dolatyar, Hamid Akbari Javar, Yaser Abdi, Ehsan Arefian, Farhood Najafi, Mehdi Doosti-Telgerd, Rahimeh B. Atashgah |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scaffold
Microfluidics microfluidic Nanofibers Pharmaceutical Science Bone Morphogenetic Protein 2 02 engineering and technology 01 natural sciences International Journal of Nanomedicine Drug Discovery Polyamines nanofibrous scaffolds bone tissue engineering Original Research Cell Death Tissue Scaffolds Chemistry Cell Differentiation General Medicine 021001 nanoscience & nanotechnology humanities Electrospinning Alkaline phosphatase 0210 nano-technology Plasmids Dendrimers Polyesters dendriplex Biophysics Bioengineering Gene delivery 010402 general chemistry Bone morphogenetic protein 2 Bone and Bones Biomaterials Tensile Strength Cell Adhesion Humans MTT assay Viability assay Particle Size Cell Shape Cell Proliferation Tissue Engineering Organic Chemistry Mesenchymal stem cell technology industry and agriculture Mesenchymal Stem Cells DNA Alkaline Phosphatase 0104 chemical sciences Durapatite Nanoparticles Calcium Biomedical engineering |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Mehdi Doosti-Telgerd,1 Fatemeh Sadat Mahdavi,2 Farzad Moradikhah,3 Mohammad Porgham Daryasari,1 Rahimeh Bayrami Atashgah,1 Banafsheh Dolatyar,4 Hamid Akbari Javar,5 Ehsan Seyedjafari,2 Iman Shabani,3 Ehsan Arefian,6 Farhood Najafi,7 Yaser Abdi,8 Mohsen Amini9 1Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 2Department of Biotechnology, University of Tehran, Tehran, Iran; 3Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran; 4Department of Cell and Developmental Biology, School of Biological Sciences, College of Science, University of Tehran, Tehran, Iran; 5Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; 6Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran; 7Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran; 8Department of Physics, University of Tehran, Tehran, Iran; 9Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranCorrespondence: Hamid Akbari JavarDepartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box: 1417614411, Tehran, IranTel +98 2164122205Email akbarijo@tums.ac.irObjective: The aim of this study is tofabricate functional scaffolds to gene delivery bone morphogenetic protein-2 (BMP-2) plasmid for bone formation in bone tissue engineering.Methods: Dendriplexes (DPs) of generation 4 polyamidoamin (G4-PAMAM)/BMP-2 plasmid were prepared through microfluidic (MF) platform. Thephysiochemical propertiesand toxicity of DPswere evaluated by DLS, AFM, FESEM and MTT assay. In order to create a suitable environment for stem cell growth and differentiation, poly-l-lactic acid (PLLA) and poly-l-lactic acid/poly (ethylene oxide) (PLLA/PEO) scaffolds containing hydroxyapatite nanoparticles (HA) and DPs were fabricated by theelectrospinning method. The osteogenic potency of the scaffolds on human adipose tissue-derived mesenchymal stem cells (hASCs) was investigated.Results: The results revealed that tuning the physical properties of DPs by adjusting flow parameters in microfluidic platform can easily improve the cell viability compared to conventional bulk mixing method. Also, the result showed that thepresence of HA and DPs in PLLA/PEO scaffold enhanced alkaline phosphatase (ALP) activity and increased the amount of deposited Ca, as well as, related toosteogenesis gen markers.Conclusion: This study indicated that onusing the MF platform in preparation of DPs and loading them along with HA in PLLA/PEO scaffold, the osteogenic differentiation of hASCs could be tuned.Keywords: microfluidic, dendriplex, nanofibrous scaffolds, bone tissue engineering |
| Databáze: | OpenAIRE |
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