Retention behaviour of some high-intensity sweeteners on different SPE sorbents
| Autor: | Andrzej Wasik, Agata Zygler, Jacek Namieśnik |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Sucralose
Chromatography Aspartame Formates Molecular Structure Formic acid Neohesperidin dihydrochalcone Solid Phase Extraction Dulcin Buffers Hydrogen-Ion Concentration Reference Standards Silicon Dioxide Analytical Chemistry chemistry.chemical_compound chemistry Sweetening Agents Neotame Ethylamines Alitame Solid phase extraction Adsorption Chromatography High Pressure Liquid |
| Zdroj: | Talanta. 82(5) |
| ISSN: | 1873-3573 |
| Popis: | The objective of this paper is to provide information about application of solid-phase extraction (SPE) for isolation of nine high-intensity sweeteners (acesulfame-K, alitame, aspartame, cyclamate, dulcin, neotame, saccharin, sucralose and neohesperidin dihydrochalcone) from aqueous solutions. The influence of several types of LC–MS compatible buffers (different pH values and compositions) on their recovery has been studied and discussed. A number of commercially available SPE cartridges, such as Chromabond C18ec, Strata-X RP, Bakerbond Octadecyl, Bakerbond SDB-1, Bakerbond SPE Phenyl, Oasis HLB, LiChrolut RP-18, Supelclean LC-18, Discovery DSC-18 and Zorbax C18 were tested in order to evaluate their applicability for the isolation of analytes. Very high recoveries (better than 92%) of all studied compounds were obtained using formic acid–N,N-diisopropylethylamine buffer adjusted to pH 4.5 and C18-bonded silica sorbents. Behaviour of polymeric sorbents strongly depends on their structure. Strata-X RP behaves much like a C18-bonded silica sorbent. Recoveries obtained using Oasis HLB were comparable with those observed for silica-based sorbents. The only compound less efficiently (83%) retained by this sorbent was cyclamate. Bakerbond SDB-1 shows unusual selectivity towards aspartame and alitame. Recoveries of these two sweeteners were very low (26 and 42%, respectively). It was also found that aspartame and alitame can be selectively separated from the mixture of sweeteners using formic acid–triethylamine buffer at pH 3.5. |
| Databáze: | OpenAIRE |
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