VEGF-PLGA controlled-release microspheres enhanced angiogenesis in encephalomyosynangiosis-based chronic cerebral hypoperfusion
| Autor: | Bin Zhao, Xiao-Yin Liu, Hong-Jun Ding, Lin Zhong, Yan Sun, Rujun Hong, Yuan-Yuan Qu, Jing-Jing Wang, Xi-Ping Yang, Mei Lu, Hong-Tao Sun, Xiao-Hong Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
CD31 Angiogenesis Polyesters medicine.medical_treatment Ischemia Collateral Circulation Neovascularization Physiologic Vascular permeability Pharmacology Brain Ischemia Neovascularization 03 medical and health sciences 0302 clinical medicine Physiology (medical) Animals Medicine Vascular Endothelial Growth Factors business.industry Endothelial Cells General Medicine Collateral circulation medicine.disease Microspheres Rats Cytokine Neurology Cerebral blood flow Cerebrovascular Circulation Delayed-Action Preparations 030220 oncology & carcinogenesis Surgery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Clinical Neuroscience. 81:122-132 |
| ISSN: | 0967-5868 |
| DOI: | 10.1016/j.jocn.2020.09.023 |
| Popis: | Treatments enhancing angiogenesis for chronic cerebral hypoperfusion (CCH) are still in the research stage. Although encephalomyosynangiosis (EMS) is a common indirect anastomosis for the treatment of CCH, the effectiveness to promote angiogenesis is not satisfactory. Vascular endothelial growth factors (VEGF) is a cytokine found to specifically act directly on vascular endothelial cells, promote neovascularization, and enhance capillary permeability. However, the short half life and unstable property of VEGF underlies the need to explore available delivery system. In this study, poly (lactide-co-glycolide) (PLGA) was used to prepare VEGF controlled-release microspheres. In vitro and in vivo analysis of release kinetics showed that the microspheres could release VEGF continuously within 30 days. Then, modified chronic cerebral hypoperfusion rat model was established by ligation of bilateral internal carotid artery and one vertebral artery. At 14 days after ischemia, the EMS and the VEGF microspheres injection were performed. At 30 days after the injection, the result of Morris water maze displayed that combinating VEGF microspheres and EMS significantly ameliorated cognitive deficit after ischemia. We observed that combinating VEGF microspheres and EMS could further significantly increase cerebral blood flow. We speculated that this enhancement of cerebral blood flow was attributed to more angiogenesis induced by combination of VEGF microspheres and EMS, which verified by more collateral circulation with cerebral angiography and higher expression of CD31 or α-SMA. Our study demonstrated that combinating VEGF-PLGA controlled-release microspheres could significantly promote angiogenesis in EMS-based CCH rats model, providing new ideas for clinical treatment of CCH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect