MMPs/TIMPs and inflammatory signalling de-regulation in human incisional hernia tissues

Autor: Maria Teresa Quiles, Jaume Reventós, Manel Armengol, Albert Sanchez-Niubo, Ramon Vilallonga, Manuel López-Cano, Santiago Ramon-y-Cajal, Pere Huguet, Maria Antonia Arbos, R. Díaz, Jordi Guillen-Marti
Přispěvatelé: Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits, Hospital Universitari Vall d'Hebron
Rok vydání: 2009
Předmět:
Male
Pathology
incisional hernia
medicine.medical_treatment
TNFA
Matrix metalloproteinase
Extracellular matrix
Pathogenesis
Càncer
Incisional hernia
Hernias
Cancer
education.field_of_study
biology
Reverse Transcriptase Polymerase Chain Reaction
Interleukin
Tissue Inhibitor of Metalloproteinases
Articles
Cáncer
Middle Aged
Matriu extracel·lular
Hernia
Abdominal
Cytokine
Enginyeria de teixits
Materials biomèdics
Molecular Medicine
Regression Analysis
Female
aponeurosis
Signal Transduction
Adult
medicine.medical_specialty
Hèrnia -- Cirurgia
extracellular matrix
Population
Enginyeria dels materials [Àrees temàtiques de la UPC]
MMP/TIMP
Gene Expression Regulation
Enzymologic
Hèrnia
Células cancerosas
medicine
Humans
Tissue engineering
RNA
Messenger
skeletal muscle
Interleukin 6
education
Aged
Inflammation
IL-6
business.industry
TNFRSFs
Cell Biology
medicine.disease
Hernia--Surgery
Matrix Metalloproteinases
biology.protein
Múscul estriat
business
Biomedical materials
Zdroj: Journal of Cellular and Molecular Medicine
UPCommons. Portal del coneixement obert de la UPC
Universitat Politècnica de Catalunya (UPC)
Recercat. Dipósit de la Recerca de Catalunya
instname
Journal Of Cellular And Molecular Medicine
r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
Fundació Sant Joan de Déu
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
ISSN: 1582-4934
1582-1838
Popis: Background: Incisional hernia is a common and important complication of laparotomies. Epidemiological studies allude to an underlying biological cause, at least in a subset of population. Interest has mainly focused on abnormal collagen metabolism. However, the role played by other determinants of extracellular matrix (ECM) composition is unknown. Aims: To date, there are few laboratory studies investigating the importance of biological factors contributing to incisional hernia development. We performed a descriptive tissue-based analysis to elucidate the possible relevance of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in association with local cytokine induction in human incisional hernia tissues. Methods: The expression profiles of MMPs, TIMPs and pro-inflammatory cytokine signalling were investigated in aponeurosis and skeletal muscle specimens taken intraoperatively from incisional hernia (n � 10) and control (n � 10) patients. Semiquantitative RT-PCR, zymography and immunoblotting analyses were done. Results: Incisional hernia samples displayed alterations in the microstructure and loss of ECM, as assessed by histological analyses. Moreover, incisional hernia tissues showed increased MMP/TIMP ratios and de-regulated inflammatory signalling (tumor necrosis factor [TNFA] and interleukin [IL]-6 tended to increase, whereas aponeurosis TNFA receptors decreased). The changes were tissue-specific and were detectable at the mRNA and/or protein level. Statistical analyses showed several associations between individual MMPs, TIMPs, interstitial collagens and inflammatory markers. Conclusions: The increment of MMPs in the absence of a counterbalance by TIMPs, together with an ongoing de-regulated inflammatory signalling, may contribute in inducing a functional defect of the ECM network by post-translational mechanisms, which may trigger abdominal wall tissue loss and eventual rupture. The notable TIMP3 protein down-regulation in incisional hernia fascia may be of pathophysiological significance. We conclude that this study may help to pinpoint novel hypotheses of pathogenesis that can lead to a better understanding of the disease and ultimately to improvement in current therapeutic approaches.
Databáze: OpenAIRE
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