Experimental Administration of Estradiol on the Colonization of Lactobacillus fermentum and Escherichia coli in the Urogenital Tract of Mice
| Autor: | Aida Pesce de Ruiz Holgado, María Rosario Rey, Clara Silva de Ruiz, María Elena Nader-Macías |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Lactobacillus fermentum Urogenital System Pharmaceutical Science medicine.disease_cause Microbiology Mice Internal medicine Escherichia coli medicine Animals Colonization Antibacterial agent Pharmacology Colony-forming unit Mice Inbred BALB C Estradiol biology General Medicine biology.organism_classification Lactobacillus medicine.anatomical_structure Endocrinology Estrogen Vagina Female Hormone |
| Zdroj: | Biological and Pharmaceutical Bulletin. 24:127-134 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.24.127 |
| Popis: | The effect of estrogen on the microbial colonization of the urogenital tract is widely discussed, mainly in regard to women with a high incidence of Urinary Tract Infections (UTI). The aim of this work was to study the effect of estradiol on the microbial colonization of lactobacilli and E. coli in mice. Female BALB/c mice were intramuscularly (i.m.) treated with beta-estradiol (one or three doses). The next day, L. fermentum was inoculated intraurethrally with three doses of 10(7) CFU (Colony Forming Units). Later, mice were challenged with uropathogenic E. coli (1 x 10(8) CFU). The hormone levels in sera increased to values 10 times higher than in control animals. Increased differentiation of desquamated vaginal cells and keratinization of the vaginal surface were also observed. The hormonal treatment produced an increased E. coli colonization in the whole tract and a higher level of L. fermentum in kidneys on the 6th day. In mice treated with hormones and lactobacilli, one dose of estradiol was enough to protect animals against the challenge with E. coli. Three doses of estradiol produced a more pronounced protection with a lower number of E. coli. No histological modifications were produced by L.fermentum, while lymphocytic proliferation at submucosal level was observed in E. coli-challenged animals. |
| Databáze: | OpenAIRE |
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