Quantitative assessment of mast cells in recurrent aphthous ulcers (RAU)

Autor: Jarkko Hietanen, Ritva Häyrinen-Immonen, Sirajedin S. Natah, Maria Malmström, Yrjö T. Konttinen
Rok vydání: 2007
Předmět:
Male
Cancer Research
Pathology
Biopsy
Cell Count
Immunoglobulin E
Basement Membrane
Cell Degranulation
Epithelium
0302 clinical medicine
Recurrence
Image Processing
Computer-Assisted
Mast Cells
Oral mucosa
Oral Ulcer
0303 health sciences
biology
medicine.diagnostic_test
Serine Endopeptidases
Degranulation
Antibodies
Monoclonal
Middle Aged
Extracellular Matrix
medicine.anatomical_structure
Connective Tissue
030220 oncology & carcinogenesis
Periodontics
Female
Stomatitis
Aphthous
Inflammation Mediators
Oral Surgery
Adult
medicine.medical_specialty
Connective tissue
Tryptase
Pathology and Forensic Medicine
03 medical and health sciences
Chymases
medicine
Humans
030304 developmental biology
Basement membrane
Lamina propria
business.industry
Mouth Mucosa
Otorhinolaryngology
biology.protein
Tryptases
business
Zdroj: Journal of Oral Pathology & Medicine. 27:124-129
ISSN: 0904-2512
DOI: 10.1111/j.1600-0714.1998.tb01927.x
Popis: Previous studies on the frequency of mast cells (MCs) in recurrent aphthous ulcers (RAU) have yielded conflicting results. Monoclonal antibodies specific for tryptase (AA1) and anti-IgE (polyclonal antibody) were used to identify density and distribution of MCs in an immunohistochemical study of RAU (n=15), induced oral traumatic ulcers (TUs) (n=9), and control clinically healthy oral mucosa (n=15). Results were quantified by means of a VIDAS image analyzer. In all sections studied, IgE-positive cells showed similar frequency and distribution to tryptase-positive MCs. In RAU lesions, numerous tryptase-positive MCs were found in the sub-epithelial lamina propria, but MC numbers in the epithelium were low and present only in some RAU biopsies. MCs were also more numerous in RAU-inflammatory infiltrates (118+/-31 cells/mm2) than those seen in TU-inflammatory infiltrates (75+/-18 cells/mm2, P
Databáze: OpenAIRE
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