Dynamized ethyl alcohol improves immune response and behavior in murine infection with Trypanosoma cruzi
| Autor: | Larissa Ciupa, Denise Lessa Aleixo, Willian N. S. Rodrigues, Érika Cristina Ferreira, Gislaine Janaina Falkowski-Temporini, Angela Rigo Portocarrero, Patricia Flora Sandri, Silvana Marques de Araújo, Fabiana Nabarro Ferraz |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Trypanosoma cruzi Immunology Male mice Inflammation Alcohol Pharmacology Biochemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immune system Statistical analyses medicine Immunology and Allergy Animals Chagas Disease Molecular Biology biology Behavior Animal Ethanol business.industry Immunity Hematology biology.organism_classification Survival Analysis 030205 complementary & alternative medicine Staining 030104 developmental biology Cytokine chemistry Cytokines medicine.symptom business |
| Zdroj: | Cytokine. 99 |
| ISSN: | 1096-0023 |
| Popis: | Aim To evaluate the effects of dynamized ethyl alcohol (Ethylicum) 6cH and 30cH in mice infected with T. cruzi. Methods In a blind, randomized and controlled assay, 63 eight-week-old, Swiss, male mice, infected with IP (1400 trypomastigotes, T. cruzi-Y-strain), were allocated into groups: CNI-non-infected (n = 12), CI-infected and non-treated (n = 17), Et6cH-infected, treated with Ethylicum 6cH (dilution 1:1012) (n = 17), Et30cH-infected, treated with Ethylicum 30cH (dilution 1:1060) (n = 17). Treatment was administered 48 h before and after infection, followed by 56 h/56 h periods, until the 9th day after infection (a.i), for 16 h. Survival and mortality were assessed until the 82nd day after infection (a.i.). TNF-α, IL-6, IL-10, IL-5 and IL-17A cytokines were assessed in serum (3–4 animals/group), at time T0 (before infection), T8 and T12 (8th and 12th a.i), using the Mouse Cytokine 20-Plex Panel Magnetic Kit (Invitrogen, USA). Inflammation was determined in heart sections (eosin-hematoxylin staining) and behavior was analyzed with ANY-maze® software. The study was approved by the Animal Ethics Committee/UEM. Statistica 8.0 and R 3.0.2 software were used for statistical analyses. Results The greater survival observed in the Et6cH group was related to decreased inflammation in heart tissue and increased IL-5 at T0 (p Conclusion The results demonstrate the beneficial effect of Ethylicum 6cH in acute murine infection by T. cruzi. |
| Databáze: | OpenAIRE |
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