Differentially-expressed genes identified by suppression subtractive hybridization in the bone marrow hematopoietic stem cells of patients with psoriasis
| Autor: | Zhenying Zhang, Zhen Yu, Xiaoming Liu, Xuejing Tan, Pan Tian, Shixin Han, Yong-Jun Piao, Suchun Hou |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Cancer Research Bone Marrow Cells Biology Severity of Illness Index Biochemistry Complementary DNA Psoriasis Gene expression Genetics medicine Humans RNA Messenger Molecular Biology Cells Cultured Gene Library Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Subtractive Hybridization Techniques cDNA library Middle Aged Hematopoietic Stem Cells medicine.disease Haematopoiesis medicine.anatomical_structure Oncology Suppression subtractive hybridization Cancer research Leukocyte Common Antigens Molecular Medicine Female Bone marrow Stem cell |
| Zdroj: | Molecular Medicine Reports. 10:479-485 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2014.2203 |
| Popis: | Psoriasis is a T cell-mediated, chronic, relapsing and inflammatory cutaneous disorder. The dysfunctional activity of T cells in patients with psoriasis is attributed to bone marrow hematopoietic stem cells (BMHSCs). To understand the pathogenic roles of BMHSCs in psoriasis, a differential gene expression analysis was performed using suppression subtractive hybridization of the BMHSCs from a patient with psoriasis and a healthy control. Using a cDNA array dot blot screening to screen 600 genes from forward- and reverse-subtracted cDNA libraries, 17 differentially-expressed sequence tags (ESTs) were identified. The genes within the ESTs were observed to be the homologs of genes that are involved in various cellular processes, including hormone signaling, RNA catabolism, protein ADP DNA base melting, transcriptional regulation, cell cycle regulation and metabolism. CD45, which was overexpressed in the psoriatic BMHSCs, was further analyzed using relative quantitative polymerase chain reaction. In addition, the levels of CD45 in the peripheral blood cells (PBCs) of the patients with psoriasis were markedly increased and closely associated with disease severity. An abnormality of hematopoietic progenitor cells, e.g., CD45 overexpression, may be transferred to PBCs via hematopoiesis, and may account for the psoriasis-inducing properties of activated T cells. |
| Databáze: | OpenAIRE |
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