Relative stability of the S2 isomers of the oxygen evolving complex of photosystem II
| Autor: | Marilyn R. Gunner, Junjun Mao, Divya Kaur, Victor S. Batista, Mikhail Askerka, Gary W. Brudvig, Krystle Reiss, Umesh Khaniya, Yingying Zhang, Witold Szejgis, Xiuhong Cai, Muhamed Amin |
|---|---|
| Přispěvatelé: | Department of Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine PHOTOSYNTHETIC WATER OXIDATION Oxygen evolving complex (OEC) Photosystem II Linear response approximation (LRA) Protonation Plant Science Oxygen-evolving complex Photochemistry 01 natural sciences Biochemistry Redox Proton transfer Catalysis 03 medical and health sciences Deprotonation Oxidation state ELECTRON-PARAMAGNETIC-RES PK(A) VALUES pK(a) Grand canonical Monte Carlo simulations CHLORIDE BINDING Chemistry FTIR DIFFERENCE SPECTROSCOPY Cell Biology General Medicine O BOND FORMATION STATE 030104 developmental biology SUBSTRATE WATER Proton affinity O-2-EVOLVING COMPLEX PROTON RELEASE 010606 plant biology & botany |
| Zdroj: | Photosynthesis Research, 141(3), 331-341. SPRINGER |
| ISSN: | 0166-8595 |
| Popis: | The oxidation of water to O-2 is catalyzed by the Oxygen Evolving Complex (OEC), a Mn4CaO5 complex in Photosystem II (PSII). The OEC is sequentially oxidized from state S-0 to S-4. The S-2 state, (Mn-III)(Mn-IV)(3), coexists in two redox isomers: S-2,S-g=2, where Mn4 is Mn-IV and S-2,S-g=4.1, where Mn1 is Mn-IV. Mn4 has two terminal water ligands, whose proton affinity is affected by the Mn oxidation state. The relative energy of the two S-2 redox isomers and the protonation state of the terminal water ligands are analyzed using classical multi-conformer continuum electrostatics (MCCE). The Monte Carlo simulations are done on QM/MM optimized S-1 and S-2 structures docked back into the complete PSII, keeping the protonation state of the protein at equilibrium with the OEC redox and protonation states. Wild-type PSII, chloride-depleted PSII, PSII in the presence of oxidized Y-Z/protonated D1-H190, and the PSII mutants D2-K317A, D1-D61A, and D1-S169A are studied at pH 6. The wild-type PSII at pH 8 is also described. In qualitative agreement with experiment, in wild-type PSII, the S-2,S-g=2 redox isomer is the lower energy state; while chloride depletion or pH 8 stabilizes the S-2,S-g=4.1 state and the mutants D2-K317A, D1-D61A, and D1-S169A favor the S-2,S-g=2 state. The protonation states of D1-E329, D1-E65, D1-H337, D1-D61, and the terminal waters on Mn4 (W1 and W2) are affected by the OEC oxidation state. The terminal W2 on Mn4 is a mixture of water and hydroxyl in the S-2,S-g=2 state, indicating the two water protonation states have similar energy, while it remains neutral in the S-1 and S-2,S-g=4.1 states. In wild-type PSII, advancement to S-2 leads to negligible proton loss and so there is an accumulation of positive charge. In the analyzed mutations and Cl- depleted PSII, additional deprotonation is found upon formation of S-2 state. |
| Databáze: | OpenAIRE |
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