Scale-up of somatic embryogenesis in alfalfa (Medicago sativa L.) I subculture and indirect secondary somatic embryogenisis

Autor:	Bryan D. McKersie, Fang-Ming Lai
Rok vydání:	1994
Předmět:	Tissue culture Somatic embryogenesis Somatic cell Callus formation Embryogenesis Botany Subculture (biology) Horticulture Medicago sativa Biology Petiole (botany)
Zdroj:	Plant Cell, Tissue and Organ Culture. 37:151-158
ISSN:	1573-5044 0167-6857
DOI:	10.1007/bf00043609
Popis:	Three methods of increasing the productivity of somatic embryogenesis in Medicago sativa L. were investigated. In the basic procedure, somatic embryos were initiated from young petioles and carried through several phases: callus formation, suspension culture, selection of the embryogenic fraction by sieving, development, maturation, desiccation and storage. The suspensions were normally separated into three fractions by sieving. Fraction I (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::fff73eaa28ade2050d070aa66b24fb36 https://doi.org/10.1007/bf00043609 Zobrazit plný text záznamu Full text from SpringerLink