Specific Electrochemiluminescence of Aptamer-Functionalized Quantum Dots with Lysozyme and Hemin as Co-Triggers
| Autor: | Ying Wan, Ji Xubo, Shengyuan Deng, Yao Chuanguang, Chenyu Zheng, Cui Hongda, Peng Xin, Song Hongxin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chemistry
Aptamer Analytical chemistry Substrate (chemistry) 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology G-quadruplex 01 natural sciences Combinatorial chemistry 0104 chemical sciences law.invention chemistry.chemical_compound law Colloidal gold Electrochemiluminescence Lysozyme 0210 nano-technology Chemiluminescence Hemin |
| Zdroj: | Chinese Journal of Chemistry. 34:331-336 |
| ISSN: | 1001-604X |
| DOI: | 10.1002/cjoc.201500886 |
| Popis: | This work proposed a label-free, cost-effective and fairly sensitive electrogenerated chemiluminescence (ECL) strategy for the specific detection of lysozyme based on the hemin/G-quadruplex hybrid. Gold nanoparticles were spread onto the chitosan thin-film as substrate to adsorb thiolated captures: a hairpin-structured DNA, integrating dual head-tail connected functional domains: one aptamer sequence for lysozyme and the other for hemin (iron(III) proto-porphyrin IX). In the presence of the both, the hairpin conformation unfolded and transformed into the hemin/G-quadruplex motif, which quenched the ECL emission of underlaid quantum dots significantly via the consumption of dissolved oxygen as endogenous coreactant. This construction enabled a wide linear response to lysozyme, ranging from 20 pg·mL−1 to 5 µg·mL−1, with a detection limit as low as 4.95 pg·mL−1 (e.g. 9.4 pmol·L−1), demonstrating the prospective utilization of DNA technologies in bioanalysis. |
| Databáze: | OpenAIRE |
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