Urokinase secretion by gastrointestinal adenocarcinoma

Autor: Elena A. Dzhenkova, Nikolay S. Samoylenko, Larisa Kozlova, Maksim N. Duritskiy, Andrey A. Maslov, Oleg I. Kit, Elena M. Frantsiyants, Yuriy A. Gevorkyan, V. I. Aleynov
Rok vydání: 2019
Předmět:
Zdroj: Journal of Clinical Oncology. 37:e15529-e15529
ISSN: 1527-7755
0732-183X
Popis: e15529 Background: The serine protease urokinase plasminogen activator (uPA) plays an important role in growth and invasion of malignant tumors. The purpose of the study was a comparative analysis of uPA activity (uPA-act) and its antigen (uPA-Ag) content in adenocarcinoma (A) of the esophagus (EA), stomach (SA), pancreatic head (PHA), sigmoid colon (SCA) and rectum (RA). Methods: Tissues of A, perifocal tissues (PT) and resection line tissues were studied by ELISA in 144 patients (39-73 years) with EA (n = 12, G2, T2-3N0-1M0), SA (n = 28, G2, T2-3N0-1M0), PHA (n = 46, G2, Т2-4N0M0), SCA (n = 31, G2, T2-3N0-1M0) and RA (n = 26, G2,T2-3N0-1M0). Statistical analysis was performed using the Statistica 10 program. Results: The levels of uPA-Ag in gastrointestinal A decreased from SA to PHA: SA˃RA˃SCA˃EA˃PHA (p < 0.01). The uPA-Ag levels in A were higher than in the resection line in all cases (p < 0.01). PT levels of uPA-Ag decreased from SA to PHA: SA˃SCA˃RA˃EA˃PHA (p < 0.01). In EA and PHA, PT levels of uPA-Ag were lower than in resection line tissues (p < 0.01), while in SA, SCA and RA they were higher (p < 0.01). Levels of uPA-act were similar in SA and SCA, while in EA and RA they were 1.2 (p = 0.4324) and 1.3 (p = 0.4012) times lower, and in PHA – 40.0-52.5 times lower (p < 0.01) compared to all A samples. The uPA-act in PT was the highest in SCA; 1.4 times lower in SA and RA; 1.7 times lower in EA; 30.9 times lower in PHA. The levels in PT were higher than in the resection line in all cases (p < 0.01). The resection line tissues in EA showed the maximal uPA-Ag content, while their uPA-act was similar to that in other tumors. Levels of uPA-Ag and uPA-act in resection line tissues in other tumors varied but were comparable. Estimated uPA-Ag/uPA-act coefficients in the resection line in SCA and RA were 20.4 and 29.5, respectively; in EA and PHA – 102.0 and 34.8, respectively. Conclusions: Adenocarcinomas of the esophagus, stomach, pancreatic head, sigmoid colon and rectum secrete uPA-Ag and uPA-act into surrounding tissues. The rate of uPA-Ag secretion by the tumor and its activation into uPA-act in pancreatic head cancer differ from that in other tumors.
Databáze: OpenAIRE
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