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Application of organic carbon to grassland in the form of manure or cattle slurry during the growing season is a normal agricultural practice. Under enhanced organic C supply changes in the grassland soil's internal N cycling and in the microbial community are expected, in particular, the fungi–bacteria ratio. Here we present novel results from a 15 N tracing study which aimed to investigate the role of fungi and bacteria in N transformations under enhanced organic C supply. We applied 15 N labelled fertiliser to soil in a laboratory incubation with and without acetate addition. Moreover, we used specific microbial inhibitors to selectively inhibit either fungi or bacteria. The data from the incubation were used to calculate gross N transformation rates via 15 N tracing models based on Monte Carlo sampling techniques. Our results show that fungi are the predominant organisms that carry out N transformations in a temperate grassland soil. Under enhanced organic C supply, the stimulation of the mineralization – immobilization – turnover and heterotrophic nitrification was predominantly related to fungal activity, indicating that fungi may play an increasingly important role in soils with an enhanced C supply. In addition, we showed that fungi were the dominant organisms for N 2 O production. Increased fungal activity under enhanced organic C supply may therefore increase N 2 O emissions from soil and have environmental consequences. |
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