Use of Horseradish Peroxidase in Identification of Serum Antibodies and Immune Complexes

Autor:	Leo P. Cawley, William M. Murphy, Sharad D. Deodhar
Rok vydání:	1973
Předmět:	Antiserum biology Chemistry Biochemistry (medical) Clinical Biochemistry Horseradish peroxidase Molecular biology Staining chemistry.chemical_compound Biochemistry Antigen biology.protein Antibody Fluorescein Fluorescein isothiocyanate Peroxidase
Zdroj:	Clinical Chemistry. 19:1370-1373
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/19.12.1370
Popis:	We made 202 examinations on specimens from 78 patients, to evaluate the application of horseradish peroxidase-labeled antisera as compared to fluorescein-labeled material in a clinical immunology laboratory. Various circulating and tissue-fixed antibodies were identified by both techniques. The horseradish peroxidase technique can be substituted for the fluorescein isothiocyanate method for the evaluation of anti-nuclear antibody and anti-mitochondrial antibody without loss of specificity or sensitivity. Although the results are comparable, fluorescein isothiocyanate appears to be slightly more sensitive in the detection of the other circulating and tissue-fixed human antibodies examined. Further refinements in preparation and technique could well lead to the fluorescein method being replaced by horseradish peroxidase staining in the future.
Databáze:	OpenAIRE
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