Effect of Deuterium on the Expression of Inducible Genes in Escherichia coli
| Autor: | Rubanovich Av, E. V. Igonina, S. V. Smirnova, S. K. Abilev |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
0106 biological sciences 0301 basic medicine DNA damage Cell medicine.disease_cause 010603 evolutionary biology 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound medicine Hydrogen peroxide Escherichia coli Incubation Gene biology biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology 030104 developmental biology medicine.anatomical_structure chemistry bacteria General Agricultural and Biological Sciences Biosensor Bacteria |
| Zdroj: | Biology Bulletin. 46:1595-1600 |
| ISSN: | 1608-3059 1062-3590 |
| Popis: | It has been shown for the first time that preliminary incubation of bacteria in a medium containing deuterium oxide (D2O) at concentrations of 2.5 to 10% leads to an increase in recA expression induced by hydrogen peroxide at concentrations of 2.2–8.8 mmol/L. The induction of recA in deuterated and non-deuterated (control) cultures was compared using a biosensor based on the E. coli strain K12 MG1655 (pRecA-lux), where luminescence occurs as a result of recA promoter activation in response to DNA damage caused by H2O2. The most effective D2O concentrations were 5.0 and 7.5%. To explain the phenomenon, expression of the catalase gene was studied in deuterated and non-deuterated cultures of E. coli K12 MG1655 (pKatG-lux) biosensor. The luminescence of this biosensor results from activation of the katG promoter in response to an increase in the concentration of H2O2 in the cell. It was found that D2O downregulated katG expression, which can lead to H2O2 accumulation, and, as a consequence, to an increase in the level of DNA damage as seen by an increase in recA expression. |
| Databáze: | OpenAIRE |
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