The transfer of cytoplasmic male sterility to winter-type oilseed rape (Brassica napus L.) by protoplast fusion

Autor:	Roger J. Kemble, Tina L. Barsby, Stephen A. Yarrow, Ian Grant
Rok vydání:	1987
Předmět:	Cell fusion biology fungi Genetic transfer Cytoplasmic male sterility Brassica food and beverages Plant Science General Medicine Protoplast biology.organism_classification Backcrossing Botany Genetics Habit (biology) Restriction fragment length polymorphism Agronomy and Crop Science
Zdroj:	Plant Science. 53:243-248
ISSN:	0168-9452
DOI:	10.1016/0168-9452(87)90161-0
Popis:	‘Polima’ cytoplasmic male sterility (cms) was transferred in one step from spring to winter lines of oilseed rape (Brassica. napus L.) by protoplast fusion. The transfer was confirmed by restriction fragment length polymorphism (RFLP) analysis of cytoplasmic DNA. Amphidiploid individuals with normal female fertility were recovered. The regenerated plants retained the winter habit. All traits studied were stable through subsequent sexual generations. The introduction of cms to winter lines by a conventional backcross normally takes 3 years; a similar alloplasmic substitution was achieved in approximately 9 months by protoplast fusion.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ffa8a069d58d323022f84f1334cb52d4 https://doi.org/10.1016/0168-9452(87)90161-0 Zobrazit plný text záznamu