Influence of transplant location on survival and proliferation of grafted human neural cell lines in adult rats

Autor:	Javier Yajeya, A.S. Riolobos, A. De La Fuente, Maria Santacana, Margarita Heredia, José María Criado
Rok vydání:	1999
Předmět:	Cerebellum Pathology medicine.medical_specialty Neocortex Tyrosine hydroxylase General Neuroscience Striatum Biology medicine.disease Transplantation surgical procedures operative medicine.anatomical_structure nervous system In vivo Cell culture Neuroblastoma Immunology medicine
Zdroj:	Neuroscience Research Communications. 24:51-60
ISSN:	1520-6769 0893-6609
DOI:	10.1002/(sici)1520-6769(199901/02)24:1<51::aid-nrc7>3.0.co;2-4
Popis:	Two human neural cell lines were examined after intracerebral transplantation in nonimmunosuppressed adult rats. BE(2)-M17 neuroblastoma cells or NT2 teratocarcinoma cells genetically modified with the tyrosine hydroxylase (TH) gene were transplanted into four brain regions: neocortex, cerebellum, striatum and amygdala. The survival and proliferation of both cell lines depended on the transplant locaiton. Grafted NT2 or M17 cells survived up to 8 weeks in the neocortex while in the cerebellum, striatum or amygdala did not. M17 cells generated tumors in 85% of the rats with striatum or amygdala grafts. M17 cells grafted into the neocortex and cerebellum ceased proliferating and survived for longer. No animals with NT2 grafts died of a tumor at any of the post-transplant times studied. M17 cells and some NT2 cells stained for TH in vitro, but the cells failed to stain for TH in vivo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ffa56308bb04b6e257242ab3a1f17647 https://doi.org/10.1002/(sici)1520-6769(199901/02)24:1<51::aid-nrc7>3.0.co Zobrazit plný text záznamu Plný text