POTENTIATION OF HEPATOCYTE SENSITIVITY TO MITOGENS BY GLUTAMIC ACID IN PRIMARY CULTURE
| Autor: | Brian I. Carr, Kaoru Hasegawa, Yasuji Miyata |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
biology DNA synthesis Long-term potentiation General Medicine Glutamic acid General Biochemistry Genetics and Molecular Biology In vitro Endocrinology medicine.anatomical_structure Cell culture Epidermal growth factor Mitogen-activated protein kinase Internal medicine Hepatocyte biology.protein medicine |
| Zdroj: | Biomedical Research. 14:243-252 |
| ISSN: | 1880-313X 0388-6107 |
| Popis: | Adult rat hepatocytes in primary culture responded to epidermal growth factor (EGF) by increased DNA synthesis. When hepatocytes were cultured in Leibovitz L-15 medium, hepatocyte response to EGF was low compared with those cultured in Williams'medium E or Koga's medium L. Furthermore, in L-15 medium, female rat hepatocytes showed almost no response to the mitogenic action of EGF compared with male rat hepatocytes. Addition of glutamic acid (1 to 20 mM) to EGF-containing L-15 medium not only enhanced DNA synthesis over 10-fold in both male and female hepatocytes, but eliminated the sex differences in DNA synthesis. Glutamic acid by itself did not stimulate DNA synthesis at any concentrations tested |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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