Autor: |
E.S. Gankina, A.Ph. Lobazov, B.G. Belen'kii, S. V. Nechaev, T.B. Adamovich, M.G. Solonenko |
Rok vydání: |
1986 |
Předmět: |
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Zdroj: |
Journal of Chromatography A. 365:315-320 |
ISSN: |
0021-9673 |
DOI: |
10.1016/s0021-9673(01)81570-3 |
Popis: |
A laser fluorometric detector for quantitative analysis of 5-dimethylaminonaphthalene-1-sulphonyl (Dns) amino acids has been developed. Photon counting was used for fluorescence detection and a computer was used for data processing. Different types of scanning were used to determine the amount of substance in a spot. Calibration curves for Dns-Ile and Dns-Gly down to 5 · 10 −14 mol per spot were obtained with an error of less than 10% for each component. Unknown quantities of Dns-Ile and Dns-Gly were determined with an accuracy of 7%. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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